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Whole-cell abundance profiling and interaction analysis of UBQLN2

ABSTRACT: To address whether expression of ALS/FTD-associated UBQLN2 mutant variants changes the cellular proteome, we performed label-free quantification-based global protein abundance profiling of patient-derived LCLs and CRISPR engineered HeLa cells. UBQLN2 mutant in both cell lines carried the mutation T487I or P497S both of which were reported to cause ALS (Deng et al., 2011; Williams et al, 2012). In addition, HeLa cells stably expressing HA-tagged MAP1B (HA-MAP1B) were subjected to HA immunoprecipitation (IP) followed by mass spectrometry to identify MAP1B interacting proteins.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Christian Behrends

LAB HEAD: Christian Behrends

PROVIDER: PXD029747 | Pride | 2022-06-24

REPOSITORIES: Pride

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			Action	DRS
	20180724_LS_21_1_A.raw	Raw
	20180724_LS_21_1_B.raw	Raw
	20180724_LS_21_1_C.raw	Raw
	20180724_LS_21_1_D.raw	Raw
	20180724_LS_21_2_A.raw	Raw

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