Proteomics

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Secretome analysis of polarised macrophages derived from induced pluripotent stem cells

ABSTRACT: Macrophages (Mɸ) are highly heterogenous and versatile innate immune cells involved in homeostatic and immune responses. Activated Mɸ can exist in two extreme phenotypes: pro-inflammatory (M1) and anti-inflammatory (M2) Mɸ and these phenotypes can be recapitulated in vitro by using lipopolysaccharide (LPS) plus IFNγ and IL-4, respectively. In the recent years, human induced pluripotent stem cells (iPSC) derived-Mɸ have gained major attention since they are functionally similar to human monocyte derived-Mɸ and are receptive to genome editing. We performed quantitative proteomics of culture supernatants to identify soluble factors released from differentially polarised iPSC-derived Mɸ (iPSDM). Our analysis suggests that the cytokine/chemokine profiles released from polarised iPSDM are similar to monocyte-derived Mɸ.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Macrophage

SUBMITTER: Gavuthami Murugesan  

LAB HEAD: Professor Paul Crocker

PROVIDER: PXD029831 | Pride | 2022-03-15

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
Deamidation__N_Sites.txt Txt
Dioxidation__MW_Sites.txt Txt
GM-M1-1-Sec.raw Raw
GM-M1-2-Sec.raw Raw
GM-M1-3-Sec.raw Raw
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Publications

Quantitative Proteomics of Polarised Macrophages Derived from Induced Pluripotent Stem Cells.

Murugesan Gavuthami G   Davidson Lindsay L   Jannetti Linda L   Crocker Paul R PR   Weigle Bernd B  

Biomedicines 20220123 2

Macrophages (M<sub>Φ</sub>) are highly heterogenous and versatile innate immune cells involved in homeostatic and immune responses. Activated M<sub>Φ</sub> can exist in two extreme phenotypes: pro-inflammatory (M1) M<sub>Φ</sub> and anti-inflammatory (M2) M<sub>Φ</sub>. These phenotypes can be recapitulated in vitro by using ligands of toll-like receptors (TLRs) and cytokines such as IFNγ and IL-4. In recent years, human induced pluripotent stem cells (iPSC)-derived M<sub>Φ</sub> have gained maj  ...[more]

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