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A MULTI-DIMENSIONAL INTERACTOMICS VIEW ON THE PLANT SNRK1 KINASE COMPLEX REVEALS A KEY REGULATORY ROLE FOR THE CLASS II T6P SYNTHASE-LIKE PROTEINS – part1 AP-MS dataset

ABSTRACT: The plant SNF1-related kinase (SnRK1) plays a central role in energy and metabolic homeostasis. SnRK1 controls growth upon activation by energy-depleting stress conditions, while also monitoring key developmental transitions and nutrient allocation between source and sink tissues. To obtain deeper insight into the SnRK1 complex and its upstream regulatory mechanisms, we explored its protein interaction landscape in a multi-dimensional setting, combining affinity purification, proximity labeling and crosslinking mass spectrometry. Integration of these analyses not only offers a unique view on the composition, stoichiometry and structure of the core heterotrimeric SnRK1 complex but also reveals a myriad of novel robust SnRK1 interactors.

INSTRUMENT(S): LTQ Orbitrap Velos, Q Exactive

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Plant Cell, Seedling, Cell Culture

SUBMITTER: Dominique Eeckhout

LAB HEAD: Geert De Jaeger

PROVIDER: PXD029833 | Pride | 2022-08-30

REPOSITORIES: Pride

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			Action	DRS
	AP-MS_experiments.xlsx	Xlsx
	Araport11plus_DE2019.fasta	Fasta
	Araport11plus_DE2020.fasta	Fasta
	E14575_2p_PSB_EXT-655_TAP_25-10-16-OBD-10.raw	Raw
	E14577_2p_PSB_EXT-655_TAP_25-10-16-OBD-11.raw	Raw

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