Proteomics

Dataset Information

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A MULTI-DIMENSIONAL INTERACTOMICS VIEW ON THE PLANT SNRK1 KINASE COMPLEX REVEALS A KEY REGULATORY ROLE FOR THE CLASS II T6P SYNTHASE-LIKE PROTEINS – part2 TurboID-MS dataset


ABSTRACT: The plant SNF1-related kinase (SnRK1) plays a central role in energy and metabolic homeostasis. SnRK1 controls growth upon activation by energy-depleting stress conditions, while also monitoring key developmental transitions and nutrient allocation between source and sink tissues. To obtain deeper insight into the SnRK1 complex and its upstream regulatory mechanisms, we explored its protein interaction landscape in a multi-dimensional setting, combining affinity purification, proximity labeling and crosslinking mass spectrometry. Integration of these analyses not only offers a unique view on the composition, stoichiometry and structure of the core heterotrimeric SnRK1 complex but also reveals a myriad of novel robust SnRK1 interactors.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Plant Cell, Cell Culture

SUBMITTER: Dominique Eeckhout  

LAB HEAD: Geert De Jaeger

PROVIDER: PXD030048 | Pride | 2022-08-30

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
Araport11plus_DE2019.fasta Fasta
Araport11plus_DE2020.fasta Fasta
E22010_A1_u_1p_PSB-224_7.raw Raw
E22012_A1_u_1p_PSB-224_8.raw Raw
E22014_A1_u_1p_PSB-224_9.raw Raw
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