Proteomics

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Investigating the interactome of TurboID-tagged RABGAP1L in the presence or absence of IFNa2 by proximity labeling proteomics

ABSTRACT: RABGAP1L, a Tre2/Bub2/Cdc16 (TBC) domain-containing protein involved in the regulation of small membrane-bound GTPases, was identified in an RNAi screen to robustly potentiate the antiviral action of Interferon (IFN) against influenza A viruses (IAVs). Functional studies revealed that the catalytically active TBC domain of RABGAP1L promotes antiviral activity. By using proximity-labeling approaches, the aim was to investigate the protein’s interactome and thereby identify its possible role in existing signaling pathways.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Disease Free

SUBMITTER: Sonja Fernbach  

LAB HEAD: Benjamin Geoffrey Hale

PROVIDER: PXD029960 | Pride | 2022-04-07

REPOSITORIES: Pride

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Dataset's files

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Action DRS
20201214_C23650_004_S280012_GFP-.raw Raw
20201214_C23650_006_S280013_GFP-IFN.raw Raw
20201214_C23650_008_S280014_WT-.raw Raw
20201214_C23650_010_S280015_WT-IFN.raw Raw
20201218_C23727_002_S280739_exp-2-GFP-.raw Raw
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Publications

Restriction factor screening identifies RABGAP1L-mediated disruption of endocytosis as a host antiviral defense.

Fernbach Sonja S   Spieler Eva E EE   Busnadiego Idoia I   Karakus Umut U   Lkharrazi Anouk A   Stertz Silke S   Hale Benjamin G BG  

Cell reports 20220301 12

Host interferons (IFNs) powerfully restrict viruses through the action of several hundred IFN-stimulated gene (ISG) products, many of which remain uncharacterized. Here, using RNAi screening, we identify several ISG restriction factors with previously undescribed contributions to IFN-mediated defense. Notably, RABGAP1L, a Tre2/Bub2/Cdc16 (TBC)-domain-containing protein involved in regulation of small membrane-bound GTPases, robustly potentiates IFN action against influenza A viruses (IAVs). Func  ...[more]

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