Dataset Information

Mice tPVAT proteome profiling using high-throughput comparative proteomic analysis based on TMT labeling

ABSTRACT: High-throughput comparative proteomic analysis based on TMT labeling combined with LC-MS/MS were performed on an in vivo Ang II infusion mice model to obtain a comprehensive view of the protein ensembles associated with thoracic PVAT (tPVAT) dysfunction induced by Ang II. In total, 5,037 proteins were confidently identified, of which 4,984 proteins were quantified. Compared with saline group, 145 proteins were upregulated and 146 proteins were downregulated during Ang II-induced tPVAT pathogenesis. Bioinformatics analyses revealed that the most enriched GO terms were annotated as gene silencing, monosaccharide binding, and extracellular matrix. In addition, some novel proteins potentially associated with Ang II infusion were identified, such as acyl-CoA carboxylase α, very long-chain acyl-CoA synthetase (ACSVL), uncoupling protein 1 (UCP1), perilipin, RAS protein-specific guanine nucleotide-releasing factor 2 (RasGRF2), and hypoxia inducible factor 1α (HIF-1α). Ang II could directly participate in the regulation of lipid metabolism, transportation, and adipocyte differentiation by affecting UCP1 and perilipin. Importantly, the key KEGG pathways were involved in fatty acid biosynthesis, FABP3-PPARα/γ, RasGRF2-ERK-HIF-1α, RasGRF2-PKC-HIF-1α, and STAT3-HIF-1α axis.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Adipose Tissue

SUBMITTER: Wenjuan Yao

LAB HEAD: Wenjuan Yao

PROVIDER: PXD029980 | Pride | 2022-02-17

REPOSITORIES: Pride

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Publications

Comparative Proteomic Analysis of tPVAT during Ang II Infusion.

Liang Xiuying X Guan Haijing H Sun Jingwen J Qi Yan Y Yao Wenjuan W

Biomedicines 20211202 12

Perivascular adipose tissue (PVAT) homeostasis plays an important role in maintaining vascular function, and PVAT dysfunction may induce several pathophysiological situations. In this study, we investigated the effect and mechanism of the local angiotensin II (Ang II) on PVAT. High-throughput comparative proteomic analysis, based on TMT labeling combined with LC-MS/MS, were performed on an in vivo Ang II infusion mice model to obtain a comprehensive view of the protein ensembles associated with ...[more]

PMID: 34944635

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