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Subcellular proteomics of murine presomitic mesoderm

ABSTRACT: We studied the effects of different stimuli on on the subcellular proteome of primary murine presomitic mesoderm tissue (PSM) in two separate experiments. In the first experiment, tissue was treated ex vivo with either fructose-6-phosphate, fructose-1,6-bisphosphate, or control treatment before subcellular fractionation using a subcellular protein fractionation kit (Thermo Fisher Scientific 78840). In the second experiment, PSMs from transgenic and control mice were extracted and subcellularly fractionated as above. Samples were multiplexed using TMT and analyzed on LC-MS/MS.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Embryo, Primary Cell, Early Embryonic Cell

SUBMITTER: Henrik Hammarén

LAB HEAD: Martin Beck

PROVIDER: PXD029988 | Pride | 2022-12-07

REPOSITORIES: Pride

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			Action	DRS
	B5006_merged_results_20190515_1152_peptides.txt	Txt
	B5006_merged_results_20190515_1152_proteins.txt	Txt
	B5007_merged_results_20190516_1741_peptides.txt	Txt
	B5007_merged_results_20190516_1741_proteins.txt	Txt
	B5012_merged_results_20190716_1407_peptides.txt	Txt

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