Proteomics

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Prostate cancer cell LNCaP_cell glycosylation_GDF15 glycosylation inducing CRPC


ABSTRACT: we examined the glycoproteomics of N-glycosylation in untreated LNCaP (NC), ST-EPI, LT-EPI, ST-ENZ, and LT-ENZ groups using Tandem Mass Tag (TMT) labels by nanoscale liquid chromatography coupled to tandem mass spectrometry (nanoLC-MS/MS).LNCaP-NC, SP-EPI, SP-ENZ, LP-EPI, and LP-ENZ cells each with two biological replicates were used for glycoproteomics analysis.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: 荣 王  

LAB HEAD: Rong Wang

PROVIDER: PXD030036 | Pride | 2022-08-12

REPOSITORIES: Pride

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Publications

N-glycosylation of GDF15 abolishes its inhibitory effect on EGFR in AR inhibitor-resistant prostate cancer cells.

Wang Rong R   Wen Piaopiao P   Yang Ganglong G   Feng Yanyan Y   Mi Yuanyuan Y   Wang Xiaoying X   Zhu Shenglong S   Chen Yong Q YQ   Chen Yong Q YQ  

Cell death & disease 20220719 7


Castration-resistance of prostate cancer is one of the most challenging clinical problems. In the present study, we have performed proteomics and glycomics using LNCaP model. Growth differentiation factor-15 (GDF15) level is increased in androgen receptor (AR) inhibitor-resistant cells and the inhibitory effect of GDF15 on epithelial growth factor receptor (EGFR) pathway is relieved by GDF15 N70 glycosylation. Interference of GDF15 (siRNA or N70Q dominant negative) or EGFR pathway (inhibitor or  ...[more]

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