Proteomics

Dataset Information

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Manganese availability alters the expression of its corresponding transporters and redox-dependent proteins but not superoxide dismutase in Lactococcus cremoris.


ABSTRACT: Growth of L. cremoris NCDO712 was not altered when manganese is omitted from its defined medium. This study was conducted in order to compare protein requirements of Lactococcus cremoris NCDO712 to grow in the presence or absence of manganese in defined medium (1% lactose). The majority of protein adaptation involves upregulation of Mn transporters and to a lesser extent various NADH-dependent proteins. Such adaptation is highly relevant for starter culture in dairy fermentation where manganese is limited and NADH homeostasis determines the formation of volatiles during long-term incubation e.g. cheese ripening.

INSTRUMENT(S): LTQ Orbitrap XL

ORGANISM(S): Lactococcus Cremoris

SUBMITTER: Sjef Boeren  

LAB HEAD: Sjef Boeren

PROVIDER: PXD030123 | Pride | 2022-05-16

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
BvO255_noMn_NCDO712_1.raw Raw
BvO256_Mn_NCDO712_1.raw Raw
BvO257_noMN_NCDO712_2.raw Raw
BvO258_Mn_NCDO712_2.raw Raw
BvO259_noMn_NCDO712_3.raw Raw
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Publications

Manganese Modulates Metabolic Activity and Redox Homeostasis in Translationally Blocked <i>Lactococcus cremoris</i>, Impacting Metabolic Persistence, Cell Culturability, and Flavor Formation.

Nugroho Avis Dwi Wahyu ADW   van Olst Berdien B   Bachtiar Stephanie Agnes SA   Boeren Sjef S   Kleerebezem Michiel M   Bachmann Herwig H  

Microbiology spectrum 20220531 3


Manganese (Mn) is an essential trace element that is supplemented in microbial media with varying benefits across species and growth conditions. We found that growth of <i>Lactococcus cremoris</i> was unaffected by manganese omission from the growth medium. The main proteome adaptation to manganese omission involved increased manganese transporter production (up to 2,000-fold), while the remaining 10 significant proteome changes were between 1.4- and 4-fold. Further investigation in translationa  ...[more]

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