Dataset Information

Regulation of cellular proteome and acetylome by catalytic and non-catalytic functions of class I histone deacetylases

ABSTRACT: Project description: Taking advantage of a newly generated model system, we examined the influence of class I HDAC catalytic and non-catalytic function on the cellular proteome and acetylome. Therefore, we generated HAP1 cell lines either lacking individual class I HDACs or expressing catalytically inactive isoforms. Using these cell lines, we investigated whether deletion or inactivation of specific class I HDACs better mimics inhibitor treated cells using isobaric labeling (TMTpro).

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Haploid Cell

SUBMITTER: Markus Hartl

LAB HEAD: Christian Seiser

PROVIDER: PXD030164 | Pride | 2022-08-16

REPOSITORIES: Pride

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			Action	DRS
	20201005_QExHFX1_RSLC1_Hess_Seiser_ext_Uni_MFPL_insol_acetylome_rep1_pool1.raw	Raw
	20201005_QExHFX1_RSLC1_Hess_Seiser_ext_Uni_MFPL_insol_acetylome_rep1_pool2.raw	Raw
	20201005_QExHFX1_RSLC1_Hess_Seiser_ext_Uni_MFPL_insol_acetylome_rep1_pool3.raw	Raw
	20201005_QExHFX1_RSLC1_Hess_Seiser_ext_Uni_MFPL_insol_acetylome_rep1_pool4.raw	Raw
	20201005_QExHFX1_RSLC1_Hess_Seiser_ext_Uni_MFPL_insol_acetylome_rep1_pool5.raw	Raw

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Publications

A toolbox for class I HDACs reveals isoform specific roles in gene regulation and protein acetylation.

Hess Lena L Moos Verena V Lauber Arnel A AA Reiter Wolfgang W Schuster Michael M Hartl Natascha N Lackner Daniel D Boenke Thorina T Koren Anna A Guzzardo Paloma M PM Gundacker Brigitte B Riegler Anna A Vician Petra P Miccolo Claudia C Leiter Susanna S Chandrasekharan Mahesh B MB Vcelkova Terezia T Tanzer Andrea A Jun Jun Qi JQ Bradner James J Brosch Gerald G Hartl Markus M Bock Christoph C Bürckstümmer Tilmann T Kubicek Stefan S Chiocca Susanna S Bhaskara Srividya S Seiser Christian C

PLoS genetics 20220822 8

The class I histone deacetylases are essential regulators of cell fate decisions in health and disease. While pan- and class-specific HDAC inhibitors are available, these drugs do not allow a comprehensive understanding of individual HDAC function, or the therapeutic potential of isoform-specific targeting. To systematically compare the impact of individual catalytic functions of HDAC1, HDAC2 and HDAC3, we generated human HAP1 cell lines expressing catalytically inactive HDAC enzymes. Using this ...[more]

PMID: 35994477

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