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A Bifunctional SPD-0090 contributes virulence of Streptococcus pneumoniae by affecting iron transport and galactose metabolism

ABSTRACT: Streptococcus pneumoniae (S.pneumoniae) is a Gram-positive bacterial pathogen that colonizes on the mucosal surfaces of the host's nasopharynx and upper respiratory tract and results in pneumonia. In order to survive and infect the host, S.pneumoniae must have obtain essential nutrients, such as transition metal ions . Our previous study had shown that the mRNA and protein levels of SPD-0090 are significantly upregulated in the ΔpiuA/ΔpiaA/ΔpitA triple mutant (three major iron transports), but its detailed biological function is unknown. In this study, we found that the knockout spd-0090 gene induced a delayed growth in the medium with different sugar sources. F urther iTRAQ quantitative proteomics studies revealed that SPD-0090 affects galactose metabolism and iron ion transport system. Then RT-qPCR and intracellular galactose content assays showed that SPD-0090 affects galactose uptake, leads to reduced galactose utilization. In addition, in vitro biochemical assays showed that SPD-0090 is a hemin transporter and affects iron uptake. Notably the knockout of spd-0090 resulted in an enhanced infection ability of S.pneumonaie to Our study reveals that the dual function of SPD-0090 plays an important role in the virulence of S.pneumoniae.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Streptococcus Pneumoniae Serotype 2 (strain D39 / Nctc 7466)

TISSUE(S): Whole Body

SUBMITTER: cao linlin

LAB HEAD: Xuesong Sun

PROVIDER: PXD030183 | Pride | 2022-06-01

REPOSITORIES: Pride

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			Action	DRS
	SPD-0090-iTRAQ-result.rar	Other
	SPD-0090_ITRAQ_1.raw	Raw
	SPD-0090_ITRAQ_2.raw	Raw
	SPD-0090_ITRAQ_3.raw	Raw
	SPD-0090_ITRAQ_4.raw	Raw

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