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TMT analysis of tissue proteomics in bevacizumab-resistant xenograft tumor tissuein nude mice

ABSTRACT: TMT proteomics was used to detect the difference in protein expression of bevacizumab-resistant xenograft tumor tissue in nude mice.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Colon

DISEASE(S): Colon Cancer

SUBMITTER: Yuning Lin

LAB HEAD: Dongmei Zhang

PROVIDER: PXD030202 | Pride | 2022-10-13

REPOSITORIES: Pride

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Publications

Targeting FAPα-expressing hepatic stellate cells overcomes resistance to antiangiogenics in colorectal cancer liver metastasis models.

Qi Ming M Fan Shuran S Huang Maohua M Pan Jinghua J Li Yong Y Miao Qun Q Lyu Wenyu W Li Xiaobo X Deng Lijuan L Qiu Shenghui S Liu Tongzheng T Deng Weiqing W Chu Xiaodong X Jiang Chang C He Wenzhuo W Xia Liangping L Yang Yunlong Y Hong Jian J Qi Qi Q Yin Wenqian W Liu Xiangning X Shi Changzheng C Chen Minfeng M Ye Wencai W Zhang Dongmei D

The Journal of clinical investigation 20221003 19

Vessel co-option has been demonstrated to mediate colorectal cancer liver metastasis (CRCLM) resistance to antiangiogenic therapy. The current mechanisms underlying vessel co-option have mainly focused on "hijacker" tumor cells, whereas the function of the "hijackee" sinusoidal blood vessels has not been explored. Here, we found that the occurrence of vessel co-option in bevacizumab-resistant CRCLM xenografts was associated with increased expression of fibroblast activation protein α (FAPα) in t ...[more]

PMID: 35951441

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Project description:Global mRNA expression profiling of patient derived pancreatic carcinoma xenograft Bo63 were collected using Agilent human whole genome array (G4845A AMADID 026652, cRNA 4x44k V2) . Two different sources of RNA were analyzed: 1.) Bo63 xenograft tumors grown on nude mice treated with vehicle only as control. 2.) Bo63 xenograft tumor grown on nude mice treated with JQ1 and SAHA (SAHA 25 mg/kg 1-0-0 and JQ1 50 mg/kg 0-0-1, treatment was imitated when tumor size reached 200 mm³ +/- 20 mm³. Two conditions (vehicle vs JQ1-SAHA treatment), each condition is represented by 3-4 biological replicates

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Transcription profiling of human A549 lung cancer cells and their isogenic mitochondrial DNA (mtDNA)-depleted rho zero counterparts grown in cell culture and as tumor xenografts in immune-deficient mice.

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