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Full length protein sequencing method based on less specific and unspecific hydrolysis strategies 2

ABSTRACT: We describe a new strategy for LC-MS/MS based full length protein sequencing. Protein samples were unspecific hydrolyzed by three different methods(proteinase K, papain and microwave-assisted acid hydrolysis) to improve overlapping degree of peptides. After LC-MS/MS analysis, peptide sequences were gernated by de novo peptide sequencing program Pnovo. A new sequence assembly program, based on de brujin graph and Overlap-Layout-Consensus strategy, was desined to generate full length protein sequence using Pnovo results.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Equus Caballus (horse) Homo Sapiens (human) Bos Taurus (bovine)

TISSUE(S): Cell Culture

SUBMITTER: zhou zhong hua

LAB HEAD: Gong Zhang

PROVIDER: PXD030203 | Pride | 2022-02-15

REPOSITORIES: Pride

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			Action	DRS
	BSA_MAAH_1.raw	Raw
	BSA_MAAH_1_HCDFT.mgf	Mgf
	BSA_MAAH_1_HCDFT_feature.csv.deepnovo_denovo	Csv
	BSA_MAAH_1_peaks.csv	Csv
	BSA_MAAH_1_pnovoCMD.res	Other

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