Bulk RNASeq of mouse astrocytes isolated from the somatosensory cortex of control and Tcf7l2 conditional knockout (TdTomato+).

ABSTRACT: The aim of the experiment was to explain the role of astrocytic TCF7L2 transcription factor in astrocyte development and maturation. To do that we used Tcf7l2FL/FL:TdTomatoFL/FL animals with loxP sites that flanked exon 6, the deletion of which causes a frameshift mutation and leads to nonsense-mediated decay. Those mice were crossed with Aldh1l1Cre-ERT2 animals, that carried a transgene with tamoxifen-inducible Cre recombinase under the control of an astrocyte lineage-specific promoter. Control line was obtained crossing Aldh1l1Cre-ERT2 with Tcf7l2WT/WT:TdTomatoFL/FL animals. Tamoxifen (75 mg/kg body weight) was administered on postnatal day 6 and 8, 27 days later, (postnatal day 35), mice were sacrificed and TdT postive astrocytes were FACS-sorted using BD FACSAria, RNA from 4 control Td

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Mus musculus

SUBMITTER: Lukasz Szewczyk 

PROVIDER: E-MTAB-13153 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress