Dataset Information

QuantitaQuantitative proteomic analysis of β-cells response to thapsigargin and rotenone uncovers key pathways of rat β-cell dysfunction tive proteomic analysis of β-cells response to thapsigargin and rotenone uncovers key pathways of rat β-cell dysfunction

ABSTRACT: We designed a TMT peptide labeling-based quantitative proteomics strategy to achieve a high-accuracy analysis of proteomic changes in response to ER stress and mitochondria stress on INS-1 cells under three different culture conditions: control (Cont), rotenone (Rot), and thapsigargin (Tpg), each with five biological replicates. The TMT data was validated using label-free quantification method.

INSTRUMENT(S): Orbitrap Exploris 240

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Insulin Secreting Cell

SUBMITTER: Mehari Weldemariam

LAB HEAD: Qibin Zhang

PROVIDER: PXD030711 | Pride | 2023-07-20

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	INS1_15spd_labelfree_Indv_400ng_15indvAND3ppol_combined.msf	Msf
	INS1_15spd_labelfree_Indv_400ng_15indvAND3ppolandlib_combined_2stage.pdResult	Other
	INS1_15spd_labelfree_Indv_400ng_Cont-1.raw	Raw
	INS1_15spd_labelfree_Indv_400ng_Cont-2.raw	Raw
	INS1_15spd_labelfree_Indv_400ng_Cont-3.raw	Raw

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Pancreatic INS-1 β-Cell Response to Thapsigargin and Rotenone: A Comparative Proteomics Analysis Uncovers Key Pathways of β-Cell Dysfunction.

Weldemariam Mehari Muuz MM Woo Jongmin J Zhang Qibin Q

Chemical research in toxicology 20220511 6

Insulin-secreting β-cells in the pancreatic islets are exposed to various endogenous and exogenous stressing conditions, which may lead to β-cell dysfunction or apoptosis and ultimately to diabetes mellitus. However, the detailed molecular mechanisms underlying β-cell's inability to survive under severe stresses remain to be explored. This study used two common chemical stressors, thapsigargin and rotenone, to induce endoplasmic reticulum (ER) and mitochondria stress in a rat insuloma INS-1 832/ ...[more]

PMID: 35544339

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