Proteomics

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Proteomic profiling of idiopathic Parkinson’s disease primary patient cells by SWATH-MS

ABSTRACT: We performed Sequential Window Acquisition of all THeoretical Mass Spectra (SWATH-MS) on primary patient olfactory neuroepithelial derived cells (ONS) from both idiopathic PD (iPD) and healthy controls and identified 228 differentially quantified proteins. Reactome pathway analysis revealed that the “Neutrophil degranulation and XBP1(S) activates chaperone genes pathways” were the most affected, indicating disruption of the secretory pathway. Upon closer inspection we identified that proteins associated with the endoplasmic reticulum and the secretory pathways were the most abundantly changed, in particular proteins associated with the unfolded protein response (UPR). In order to validate this finding, we performed qRT-PCR analysis on patient ONS subjected to ER stressors and confirmed that the iPD patient cells had elevated UPR responses, in particular to tunicamycin mediated ER stress.

INSTRUMENT(S): TripleTOF 6600

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

DISEASE(S): Parkinson's Disease

SUBMITTER: Peter Hains  

LAB HEAD: Peter Hains

PROVIDER: PXD030723 | Pride | 2022-05-20

REPOSITORIES: Pride

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Action DRS
100_VW_SwTuner_6600.txt Txt
C_Pool_7_01.wiff Wiff
C_Pool_7_01.wiff.scan Wiff
C_Pool_7_02.wiff Wiff
C_Pool_7_02.wiff.scan Wiff
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Proteomic profiling of idiopathic Parkinson's disease primary patient cells by SWATH-MS.

Wood Stephen A SA   Hains Peter G PG   Muller Arnaud A   Hill Melissa M   Premarathne Susitha S   Murtaza Mariyam M   Robinson Phillip J PJ   Mellick George D GD   Sykes Alex M AM  

Proteomics. Clinical applications 20220607 5

<h4>Purpose</h4>Parkinson's disease (PD) is the second most prevalent neurodegenerative disease. It is generally diagnosed clinically after the irreversible loss of dopaminergic neurons and no general biomarkers currently exist. To gain insight into the underlying cellular causes of PD we aimed to quantify the proteomic differences between healthy control and PD patient cells.<h4>Experimental design</h4>Sequential Window Acquisition of all THeoretical Mass Spectra was performed on primary cells  ...[more]

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