Proteomics

Dataset Information

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HOXB13 WT and G84E mass spectrometry

ABSTRACT: To identify potential cofactors of HOXB13 in suppressing lipogenic programs in prostate cancer cells, we performed tandem affinity purification followed by mass spectrometry analysis of WT and G84E HOXB13 expressed in LNCaP cells. Out of the HOXB13-enriched proteins are previously reported interactors such as AR and its cofactors FOXA1, GATA2, and NKX3. However, these interactions were not disrupted by G84E as compared to WT HOXB13. Interestingly, we found strong interactions of HOXB13 with HDAC1/3 and their corepressors NCoR1/2 and TBL1X. Notably, these interactions were drastically reduced by G84E mutation.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

DISEASE(S): Prostate Adenocarcinoma

SUBMITTER: Xiaodong Lu  

LAB HEAD: Jindan Yu

PROVIDER: PXD030810 | Pride | 2023-03-11

REPOSITORIES: Pride

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Dataset's files

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Action DRS
JYMS031_HOXB13_WT.pep.xml Pepxml
JYMS031_HOXB13_WT.raw Raw
JYMS032_HOXB13_G84E.pep.xml Pepxml
JYMS032_HOXB13_G84E.raw Raw
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Publications

HOXB13, a homeodomain transcription factor, critically regulates androgen receptor (AR) activities and androgen-dependent prostate cancer (PCa) growth. However, its functions in AR-independent contexts remain elusive. Here we report HOXB13 interaction with histone deacetylase HDAC3, which is disrupted by the HOXB13 G84E mutation that has been associated with early-onset PCa. Independently of AR, HOXB13 recruits HDAC3 to lipogenic enhancers to catalyze histone deacetylation and suppress lipogenic  ...[more]

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