Dataset Information

Multi-omic analyses reveal interaction networks linking CIC to cell cycle regulation

ABSTRACT: CIC encodes a transcriptional repressor inactivated by loss-of-function mutations in several cancer types, indicating that it may function as a tumor suppressor. Recent data indicate that CIC may regulate cell cycle genes in humans; however, a thorough investigation of this proposed role has not yet been reported. Here, we used single-cell RNA sequencing technology to provide evidence that inactivation of CIC in human cell lines resulted in transcriptional dysregulation of genes involved in cell cycle control. We also mapped CIC’s protein-protein and genetic interaction networks, identifying interactions between CIC and members of the Switch/Sucrose Non-Fermenting (SWI/SNF) complex, as well as novel candidate interactions between CIC and cell cycle regulators. We further showed that CIC loss was associated with an increased frequency of mitotic defects in human cell lines and a mouse model. Overall, our study positions CIC as a cell cycle regulator and indicates that CIC loss can lead to mitotic errors, consistent with CIC’s emerging role as a tumor suppressor of relevance in several cancer contexts.

INSTRUMENT(S): Orbitrap Fusion, Q TRAP

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Christopher Hughes

LAB HEAD: Gregg Morin

PROVIDER: PXD030942 | Pride | 2023-06-30

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20120131_MS0196_MM_CIC-endog-IP_Nuc_117KDa.wiff	Wiff
	20120131_MS0196_MM_CIC-endog-IP_Nuc_117KDa.wiff.scan	Wiff
	20120131_MS0196_MM_CIC-endog-IP_Nuc_130KDa.wiff	Wiff
	20120131_MS0196_MM_CIC-endog-IP_Nuc_130KDa.wiff.scan	Wiff
	20120131_MS0196_MM_CIC-endog-IP_Nuc_150KDa.wiff	Wiff

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Publications

Multi-Omic Analysis of CIC's Functional Networks Reveals Novel Interaction Partners and a Potential Role in Mitotic Fidelity.

Takemon Yuka Y LeBlanc Véronique G VG Song Jungeun J Chan Susanna Y SY Lee Stephen Dongsoo SD Trinh Diane L DL Ahmad Shiekh Tanveer ST Brothers William R WR Corbett Richard D RD Gagliardi Alessia A Moradian Annie A Cairncross J Gregory JG Yip Stephen S Aparicio Samuel A J R SAJR Chan Jennifer A JA Hughes Christopher S CS Morin Gregg B GB Gorski Sharon M SM Chittaranjan Suganthi S Marra Marco A MA

Cancers 20230517 10

<i>CIC</i> encodes a transcriptional repressor and MAPK signalling effector that is inactivated by loss-of-function mutations in several cancer types, consistent with a role as a tumour suppressor. Here, we used bioinformatic, genomic, and proteomic approaches to investigate CIC's interaction networks. We observed both previously identified and novel candidate interactions between CIC and SWI/SNF complex members, as well as novel interactions between CIC and cell cycle regulators and RNA process ...[more]

PMID: 37345142

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