Rabbit haemorrhagic disease virus (RHDV) infected rabbit liver proteome
Ontology highlight
ABSTRACT:
INSTRUMENT(S): LTQ Orbitrap
ORGANISM(S): Oryctolagus Cuniculus Cuniculus
TISSUE(S): Hepatocyte, Liver, Epithelial Cell
SUBMITTER: 
Elena Smertina
LAB HEAD: Tanja Strive
PROVIDER: PXD031027 | Pride | 2022-10-14
REPOSITORIES: Pride
Dataset's files
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Publications
Lagovirus Non-structural Protein p23: A Putative Viroporin That Interacts With Heat Shock Proteins and Uses a Disulfide Bond for Dimerization.
Smertina Elena E Carroll Adam J AJ Boileau Joseph J Emmott Edward E Jenckel Maria M Vohra Harpreet H Rolland Vivien V Hands Philip P Hayashi Junna J Neave Matthew J MJ Liu Jian-Wei JW Hall Robyn N RN Strive Tanja T Frese Michael M
Frontiers in microbiology 20220707
The exact function(s) of the lagovirus non-structural protein p23 is unknown as robust cell culture systems for the <i>Rabbit haemorrhagic disease virus</i> (RHDV) and other lagoviruses have not been established. Instead, a range of <i>in vitro</i> and <i>in silico</i> models have been used to study p23, revealing that p23 oligomerizes, accumulates in the cytoplasm, and possesses a conserved C-terminal region with two amphipathic helices. Furthermore, the positional homologs of p23 in other cali ...[more]
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2010-07-23 | E-GEOD-23076 | biostudies-arrayexpress
Project description:The mechanisms through which cardioplegia affords cardioprotection is complex and can not be investigated as a single entity but rather must be investigated as a system. In order to improve understanding of the molecular processes modulating the effects of global ischemia and the cardioprotection afforded by cardioplegia we have constructed rabbit heart cDNA libraries and have isolated, sequenced and identified a compendium of non-redundant cDNAs for use in microarray analysis. Methods: New Zealand White rabbits were used for Langendorff perfusion consisting of 30 minutes global ischemia (GI)and 120 minutes reperfusion. Cardioplegia hearts received cardioplegia prior to GI. Control hearts were perfused without GI. The effects of RNA and protein synthesis on the cardioprotection afforded by cardioplegia were investigated separately by pre-perfusion with either alpha-amanitin or cycloheximide. Rabbit heart cDNA libraries were constructed and cDNAs isolated and identified by 5’ sequencing to create a compendium of non-redundant cDNAs for microarray analysis coupled with proteomic analysis. Results and Conclusion: Our studies demonstrate that the cardioprotection afforded by cardioplegia is modulated by RNA and protein dependent mechanisms. Microarray and proteomic enrichment analysis indicated that cardioplegia significantly increases differentially expressed genes/proteins associated with the mitochondrion and mitochondrial function and significantly up-regulates the biological processes of muscle contraction, involuntary muscle contraction, carboxylic acid and fatty acid catabolic processes, fatty acid beta-oxidation and fatty acid metabolic processes.
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