Proteomics

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Pan-claudin family interactome analysis reveals shared and specific interactions

ABSTRACT: Interactome study of the pan-claudin family by combining two complementary pull-down techniques followed by mass spectrometry to create an interaction landscape of the claudin family. Co-immunoprecipitation (CoIP) of recombinant claudins expressed in MDCK-C7 cells provided information about interactions beyond the already known TJ proteins. A protein interaction screen on a peptide matrix (PRISMA) allowed mapping interactions along the disordered cytosolic C-terminal region of claudins and studying the effect of post-translational modifications (PTMs) on these interactions. The new interaction partners of the C-terminus of several claudins were confirmed by proximity ligation assays (PLA) and revealed their possible implications in localized biological processes in epithelial cells. By combining these approaches, we created an extended interaction map of the claudin protein family, expanding the current knowledge in the field.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human) Canis Familiaris (dog) (canis Lupus Familiaris)

TISSUE(S): Epithelial Cell

SUBMITTER: Lorena Suarez-Artiles  

LAB HEAD: Gunnar Dittmar

PROVIDER: PXD031094 | Pride | 2023-10-24

REPOSITORIES: Pride

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Animal_20190423_LSA_CL_CoIP_Cldn11_1.raw Raw
Animal_20190423_LSA_CL_CoIP_Cldn11_2.raw Raw
Animal_20190423_LSA_CL_CoIP_Cldn11_3.raw Raw
Animal_20190423_LSA_CL_CoIP_Cldn11_4.raw Raw
Animal_20190423_LSA_CL_CoIP_Cldn12_1.raw Raw
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Publications

Claudins are a family of transmembrane proteins expressed in epithelial tissues and are the major components of tight junctions (TJs), which define barrier properties in epithelia and maintain cell polarity. How claudins regulate the formation of TJs and which functions they exert outside of them is not entirely understood. Although the long and unstructured C-terminal tail is essential for regulation, it is unclear how it is involved in these functions beyond interacting with TJ-associated prot  ...[more]

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