Dataset Information

Identification of novel constituents of ALIS from macrophage lysates (LPS+/-), LC MS/MS

ABSTRACT: Macrophages assemble aggresome-like induced structures or ALIS upon LPS or E. coli mediated TLR4 stimulation. These structures are positive for p62, LC3 and ubiquitinated proteins, form irrespective of autophagy, and persist within the cell system up to 48h. However, their composition is not well known. Our aim was to identify proteins constituting ALIS and establish its functionality in host-defense response under infectious conditions.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture, Macrophage

SUBMITTER: Srinivasa Srinivasula

LAB HEAD: Srinivasa Murty Srinivasula

PROVIDER: PXD031163 | Pride | 2024-05-23

REPOSITORIES: Pride

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TLR-mediated aggresome-like induced structures comprise antimicrobial peptides and attenuate intracellular bacterial survival.

Bhatnagar Anushree A Chopra Umesh U Raja Sebastian S Das Krishanu Dey KD Mahalingam S S Chakravortty Dipshikha D Srinivasula Srinivasa Murty SM

Molecular biology of the cell 20240103 3

Immune cells employ diverse mechanisms for host defense. Macrophages, in response to TLR activation, assemble aggresome-like induced structures (ALIS). Our group has shown TLR4-signaling transcriptionally upregulates p62/sequestome1, which assembles ALIS. We have demonstrated that TLR4-mediated autophagy is, in fact, selective-autophagy of ALIS. We hypothesize that TLR-mediated autophagy and ALIS contribute to host-defense. Here we show that ALIS are assembled in macrophages upon exposure to dif ...[more]

PMID: 38170582

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			Action	DRS
	66534-_1_.mzML	Mzml
	66534-_1_.pdResult	Other
	All_Proteins.mgf	Mgf
	LPS_treated_fraction_25.raw	Raw
	LPS_treated_fraction_26.raw	Raw