Proteomics

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Proteome and Secretome Analysis Reveals Differential Post-Transcriptional Regulation of Toll-Like Receptor Responses

ABSTRACT: Toll-like receptors (TLRs) are essential sensors in innate immunity and among the first to detect invading pathogens. Many studies of TLR responses have focused on the intracellular signaling events that occur upon receptor stimulation. However, proteins released from the cell play a key role in cell-cell communication during an immune response. We have used mass spectrometry-based proteomic methods to investigate both the intracellular and extracellular responses of macrophages stimulated with individual TLR2, TLR4, and TLR7 ligands and whole pathogens. We performed global quantitative analyses of the mouse macrophage proteome and secretome using stable isotope labeling with amino acids and high-resolution mass spectrometry.

OTHER RELATED OMICS DATASETS IN: PRJNA338478

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Macrophage

SUBMITTER: Aleksandra Nita-Lazar  

LAB HEAD: Aleksandra Nita-Lazar

PROVIDER: PXD004113 | Pride | 2017-03-06

REPOSITORIES: Pride

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Proteome and Secretome Analysis Reveals Differential Post-transcriptional Regulation of Toll-like Receptor Responses.

Koppenol-Raab Marijke M   Sjoelund Virginie V   Manes Nathan P NP   Gottschalk Rachel A RA   Dutta Bhaskar B   Benet Zachary L ZL   Fraser Iain D C ID   Nita-Lazar Aleksandra A  

Molecular & cellular proteomics : MCP 20170224 4 suppl 1

The innate immune system is the organism's first line of defense against pathogens. Pattern recognition receptors (PRRs) are responsible for sensing the presence of pathogen-associated molecules. The prototypic PRRs, the membrane-bound receptors of the Toll-like receptor (TLR) family, recognize pathogen-associated molecular patterns (PAMPs) and initiate an innate immune response through signaling pathways that depend on the adaptor molecules MyD88 and TRIF. Deciphering the differences in the com  ...[more]

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