Hage et.al Mus musculus lung LC-MSMS
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ABSTRACT: The importance of unanchored Ub in innate immunity has been shown only for a limited number of unanchored Ub-interactors. We investigated what additional cellular factors interact with unanchored Ub and whether unanchored Ub plays a broader role in innate immunity. To identify unanchored Ub-interacting factors from murine lungs, we used His-tagged recombinant poly-Ub chains as bait. These chains were mixed with lung tissue lysates and protein complexes were isolated with Ni-NTA beads. Sample elutions were subjected to mass spectrometry (LC-MSMS) analysis.
INSTRUMENT(S): LTQ Orbitrap Velos Pro
ORGANISM(S): Mus Musculus (mouse)
TISSUE(S): Lung
SUBMITTER: Adam Hage
LAB HEAD: Ricardo Rajsbaum
PROVIDER: PXD031365 | Pride | 2022-02-28
REPOSITORIES: Pride
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