Proteomics

Dataset Information

0

Arabidopsis whole seedling LC-MS/MS


ABSTRACT: Pollen grains are coated with a pollen wall which protects the microspore from various biotic and abiotic stresses, as well as facilitates male-female interaction. During pollen wall development, many compounds are transported from the tapetum to the developing pollen wall via tapetum-specific membrane transporters, and male sterility is often observed when membrane transporter’s function or localization is altered. Here, we show that mutating AP1/2β1 and AP1/2β2, two homologous genes of AP1/2β which encodes a large subunit shared by adaptor protein 1 (AP-1) and adaptor protein 2 (AP-2) complexes in Arabidopsis, impair pollen exine formation and cause reduced pollen germination rate and slowed pollen tube growth. However, the shared theory of AP1/2β is not proved in Arabidopsis.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Whole Body

SUBMITTER: Chan Liu  

LAB HEAD: Chao Wang

PROVIDER: PXD031514 | Pride | 2022-10-14

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
Data_processing_details.rar Other
RAW.rar Other
comparison_between_groups-LFQ.rar Other
comparison_within_groups-LFQ.rar Other
filtered by 1% FDR-comparison between groups-LFQ.rar Other
Items per page:
1 - 5 of 9
altmetric image

Publications

AP1/2β-mediated exocytosis of tapetum-specific transporters is required for pollen development in Arabidopsis thaliana.

Liu Chan C   Li Zhimin Z   Tian Dan D   Xu Mei M   Pan Jianwei J   Wu Haijun H   Wang Chao C   Otegui Marisa S MS  

The Plant cell 20220901 10


AP-1 and AP-2 adaptor protein (AP) complexes mediate clathrin-dependent trafficking at the trans-Golgi network (TGN) and the plasma membrane, respectively. Whereas AP-1 is required for trafficking to plasma membrane and vacuoles, AP-2 mediates endocytosis. These AP complexes consist of four subunits (adaptins): two large subunits (β1 and γ for AP-1 and β2 and α for AP-2), a medium subunit μ, and a small subunit σ. In general, adaptins are unique to each AP complex, with the exception of β subuni  ...[more]

Similar Datasets

2021-03-06 | PXD019291 | Pride
2023-03-30 | PXD037091 | Pride
2014-04-08 | E-MTAB-2334 | biostudies-arrayexpress
2023-08-04 | PXD043940 | Pride
2015-11-04 | E-GEOD-72972 | biostudies-arrayexpress
2019-03-29 | PXD013095 | Pride
2009-10-10 | GSE18325 | GEO
2012-07-30 | E-GEOD-35644 | biostudies-arrayexpress
2016-03-01 | E-GEOD-68884 | biostudies-arrayexpress
2021-03-17 | PXD023983 | Pride