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Proteomic profiling of Lemna minor exposed to Bentazon

ABSTRACT: Lemna minor a small aquatic plant has been used extensively in ecotoxicolgical testing to elucidate substance-related effects to freshwater plants. They are free-floating freshwater macrophyte, very sensitive towards chemical exposure and easy to cultivate thus makes the plant suitable for laboratory testing. Here we present a rapid and reproducible data dependent proteomics approach for identifying growth related molecular signatures in lemna minor as an alternative to algae testing. For this, we have analyzed the proteome of lemna minor exposed to bentazon as a model substances for identifying growth related molecular perturbations. These fingerprints allow for a definition of potential biomarkers as tools in screening approaches and for integration in plant growth inhibition studies, for identifying suspect substances, such as in the Lemna sp. growth inhibition test (OECD TG 221).

INSTRUMENT(S): Q Exactive

ORGANISM(S): Lemna Minor

TISSUE(S): Plant, Leaf

SUBMITTER: Steve Ayobahan

LAB HEAD: Dr. Sebastian Eilebrecht

PROVIDER: PXD031679 | Pride | 2022-08-15

REPOSITORIES: Pride

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			Action	DRS
	TMT_109_2_001.raw	Raw
	TMT_109_2_002.raw	Raw
	TMT_109_2_003.raw	Raw
	TMT_110_2_001.raw	Raw
	TMT_110_2_002.raw	Raw

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Proteomic profiling of Lemna minor exposed to Atorvastatin

Project description:Lemna minor a small aquatic plant has been used extensively in ecotoxicolgical testing to elucidate substance-related effects to freshwater plants. They are free-floating freshwater macrophyte, very sensitive towards chemical exposure and easy to cultivate thus makes the plant suitable for laboratory testing. Here we present a rapid and reproducible data dependent proteomics approach for identifying growth related molecular signatures in lemna minor as an alternative to algae testing. For this, we have analyzed the proteome of lemna minor exposed to atorvastatin as a model substances for identifying growth related molecular perturbations. These fingerprints allow for a definition of potential biomarkers as tools in screening approaches and for integration in plant growth inhibition studies (OECD TG 221), for identifying suspect substances.

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