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Differential centrifugation coupled to affinity purification-mass spectrometry of mcherry-ATG8E

ABSTRACT: Autophagosomes are double-membraned vesicles that traffic harmful or unwanted cellular macromolecules to the vacuole for recycling. Although autophagosome biogenesis has been studied extensively, autophagosome maturation, i.e., delivery and fusion with the vacuole, remains largely unknown in plants. To start filling these gaps, we devised a differential centrifugation set up, where we enriched intact autophagosomes and performed affinity purification-mass spectrometry (AP-MS), using ATG8 as a bait. This method enabled the identification of an autophagy adaptor protein.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Whole Plant, Mixture

SUBMITTER: Richard Imre

LAB HEAD: Yasin Dagdas

PROVIDER: PXD031787 | Pride | 2022-10-18

REPOSITORIES: Pride

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			Action	DRS
	20211221_20220111_E3_Bui_Dagdas_GMI_ID568_584_onbead_first8samples_TAIR_Step2_iBAQ_Limma_12ratios.pdResult	Other
	20211221_E3_RSLC3_Bui_Dagdas_GMI_ID568_onbead_dig_30per_1_control_mCherry_S4.raw	Raw
	20211221_E3_RSLC3_Bui_Dagdas_GMI_ID568_onbead_dig_30per_2_control_mCherry_S4_protease.raw	Raw
	20211221_E3_RSLC3_Bui_Dagdas_GMI_ID568_onbead_dig_30per_3_control_mCherry_P4.raw	Raw
	20211221_E3_RSLC3_Bui_Dagdas_GMI_ID568_onbead_dig_30per_4_control_mCherry_P4_protease.raw	Raw

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