Proteomics

Dataset Information

0

Conoid extrusion serves as gatekeeper for entry of glideosome components into the pellicular space to control motility and invasion in Apicomplexa


ABSTRACT: Members of the apicomplexans are defined by apical cytoskeletal structures and secretory or-ganelles, tailored for motility and invasion. Gliding is powered by the actomyosin-dependent rearward translocation of apically secreted transmembrane adhesins. In Toxoplasma gondii, the conoid, composed of a cone of spiraling tubulin fibers and apposed preconoidal rings (PCRs), is an enigmatic, dynamic organelle of undefined function. Here we mapped five new components of the PCRs and deduce that the structure serves as a pivotal hub for actin polymerization and glideosome assembly. F-actin produced by Formin1 on the PCRs is used by Myosin H to generate the force for conoid extrusion. A set of conserved B-box-type zinc finger domain containing proteins in Apicomplexa is indispensable for PCRs formation, co-noid extrusion and motility in Toxoplasma and Plasmodium. Conoid dynamics directs the flux of F-actin to the pellicular space, acting as dynamic gatekeeper to tightly control parasite motility during invasion and egress.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Toxoplasma Gondii Gt1

SUBMITTER: Oscar Vadas  

LAB HEAD: Oscar Vadas

PROVIDER: PXD031816 | Pride | 2023-08-31

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
20210317_OT_HDXMS35.raw Raw
20211011_OT_HDXMS_06.raw Raw
20211011_OT_HDXMS_07.raw Raw
20211011_OT_HDXMS_08.raw Raw
20211011_OT_HDXMS_09.raw Raw
Items per page:
1 - 5 of 13
altmetric image

Publications

Conoid extrusion regulates glideosome assembly to control motility and invasion in Apicomplexa.

Dos Santos Pacheco Nicolas N   Brusini Lorenzo L   Haase Romuald R   Tosetti Nicolò N   Maco Bohumil B   Brochet Mathieu M   Vadas Oscar O   Soldati-Favre Dominique D  

Nature microbiology 20220915 11


Members of Apicomplexa are defined by apical cytoskeletal structures and secretory organelles, tailored for motility, invasion and egress. Gliding is powered by actomyosin-dependent rearward translocation of apically secreted transmembrane adhesins. In the human parasite Toxoplasma gondii, the conoid, composed of tubulin fibres and preconoidal rings (PCRs), is a dynamic organelle of undefined function. Here, using ultrastructure expansion microscopy, we established that PCRs serve as a hub for g  ...[more]

Similar Datasets

2023-05-10 | PXD039335 | Pride
2023-11-24 | E-MTAB-12856 | biostudies-arrayexpress
2015-02-11 | ST000282 | MetabolomicsWorkbench
2010-12-27 | E-GEOD-20480 | biostudies-arrayexpress
| S-EPMC6138688 | biostudies-literature
| S-EPMC5316484 | biostudies-literature
2023-01-30 | PXD035654 | Pride
2024-07-12 | PXD040598 | Pride
2013-07-30 | E-GEOD-42587 | biostudies-arrayexpress
2010-12-27 | GSE20480 | GEO