Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Physiological and proteomic analyses indicate delayed sowing improves photosynthetic capacity in wheat flag leaves under heat stress

ABSTRACT: Background and aims Climate warming has become an indisputable fact, and wheat is among the most heat-sensitive cereal crops. Heat stress during grain filling threatens global wheat production and food security. Here, we analyzed the physiological and proteomic changes by delayed sowing on the photosynthetic capacity of winter wheat leaves under heat stress. Our aim is to provide a new cultivation way for the heat stress resistance in wheat. Methods Through 2 years field experiment and an open warming simulation system, we compared the changes in wheat grain weight, yield, photosynthetic rate, and chlorophyll fluorescence parameters under heat stress at late grain–filling stage during normal sowing and delayed sowing. At the same time, based on the iTRAQ proteomics, we compared the changes of differentially expressed proteins (DEPs) during the two sowing periods under high temperature stress.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Wheat Dwarf Virus - [taiyuan]

TISSUE(S): Leaf

SUBMITTER: Xianliang Ma

LAB HEAD: He MingRong

PROVIDER: PXD031871 | Pride | 2022-02-26

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	Fusion_20190422_60min_mai_1-1.raw	Raw
	Fusion_20190422_60min_mai_1-2.raw	Raw
	Fusion_20190422_60min_mai_1-3.raw	Raw
	Fusion_20190422_60min_mai_10-1.raw	Raw
	Fusion_20190422_60min_mai_10-2.raw	Raw

Items per page:

1 - 5 of 46

Similar Datasets

Small RNA, transcriptome and degradome analysis of the transgenerational heat stress response network in durum wheat

Project description:Heat stress is a major limiting factor for grain yield and grain quality in wheat production. In crops, abiotic stresses have transgenerational effects and the mechanistic basis of stress memory is associated with epigenetic regulation. The current study presents the first systematic analysis of the transgenerational effects of post-anthesis heat stress in tetraploid wheat. Genotype-dependent response patterns to parental and progeny heat stress were found for the leaf physiological traits, harvest components, and grain quality traits measured. Parental heat stress had positive influence on the offspring under re-occurring stress for traits like chlorophyll content, grain weight, grain number and grain total starch content. Integrated sequencing analysis of the small RNAome, mRNA transcriptome, and mRNA degradome provided the first description of the molecular networks mediating heat stress adaption under transgenerational influence. The expression profile of 1771 microRNAs (733 being novel) and 66,559 genes was provided, with differentially expressed microRNAs and genes identified subject to the progeny treatment, parental treatment and tissue type factors. Gene Ontology and KEGG pathway annotation of stress responsive microRNAs-mRNA modules provided further information on their functional roles in biological processes like hormone homeostasis, signal transduction, and protein stabilization. Our results provide new sights on the molecular basis of transgenerational heat stress adaptation, which can be used for improving thermos-tolerance in breeding.

2021-05-24 | GSE171303 | GEO

Transcriptomic and physiological response of durum wheat grain to short period of heat stress at the early grain-filling stage

Project description:Wheat is one of the most significant crops in terms of human consumption in the world. In a climate change scenario, extreme weather event such as heatwaves will be more frequent especially during the grain-filling (GF) stage and could affect grain weight and quality of crops. Molecular mechanisms underlying the response to short heat stress (HS) have been widely reported for the hexaploid wheat (Triticum aestivum) but the regulatory heat stress mechanisms in tetraploid durum wheat (Triticum turgidum ssp. durum) remain partially understood. In this work, we performed a transcriptomic analysis of durum wheat grains to HS during early GF to identify key HS response genes and their predicted regulatory networks under glasshouse conditions.

2021-10-26 | GSE186472 | GEO

Effect of heat stress on developing wheat grain

Project description:effect of post anthesis heat stress on transcriptome of developing wheat grain

2019-12-19 | E-MTAB-8520 | biostudies-arrayexpress

Differential gene expression studies for identifying genes imparting heat stress tolerance in wheat during grain filling.

Project description:Heat stress is one of the major abiotic stress factor that affects wheat yield. Especially, heat stress during grain filling affects grain yield besides reduced grain quality. So, in our present study, three genotypes with varied levels of tolerance to heat stress were chosen. They were subjected to heat stress at two stages for three days viz., early (11-14days-post-anthesis) and late (27-30dpa) grain filling independently under controlled conditions. At 14 and 30dpa, the spikes were harvested from control and stress conditions from all the three genotypes, grains were isolated and pulverized. Hence pulverized tissues are used for RNA extraction and further for transcriptome sequencing using HiSeq 4000. Data were analyzed to identify the genes involved in imparting heat stress tolerance.

2019-12-16 | E-MTAB-6140 | biostudies-arrayexpress

Global crotonylatome and function analysis of lysine-crotonylated proteins in wheat response to cold stress

Project description:Abstract Lysine crotonylation (Kcr) is a recently identified post-translational modification (PTM) in the prokaryotic and eukaryotic organisms. However, the function of lysine-crotonylated proteins in response to abiotic stress in plants remains largely unknown. Here, we report the first global profiling of the Kcr proteome in common wheat, identifying 4,696 Kcr sites on 1,726 substrate proteins, participating in a wide variety of biological and metabolic pathways. Combination of transcriptome and proteome in a RIL (recombinant inbred line) population, Kcr proteome, and genome-wide association study, a candidate gene phosphoglycerate kinase (TaPGK) was identified to potentially affect on cold tolerance in common wheat. Using EMS-mutants, overexpressed transgenic wheat plants and CRISPR/Cas9-mediated know-out mutants, we demonstrated that TaPGK played a positively key role in regulation of plant cold tolerance. Moreover, TaPGK protein strongly interacted with sirtuin-like (TaSRT1). EMS-mutant tetraploid and overexpressed transgenic wheat experiments confirmed that TaSRT1 gene negatively regulated wheat cold tolerance. Immunoprecipitation demonstrated that TaSRT1 promoted the degradation of TaPGK by erasing Kcr. Additionally, RNA-sequencing results showed that overexpressed TaPGK significantly improved expression of peroxidase genes. It concluded that a large number of Kcr sites were present in wheat important proteins and TaPGK crotonylated by TaSRT1 played a key role in wheat response to cold stress through scavenging the accumulation of ROS.

2023-07-20 | PXD030768 | Pride

ANGPTL4 binds to leptin receptor to regulate ectopic bone formation and fat metabolism

Project description:Leptin protein was thought to be unique to leptin receptor (LepR), but the phenotypes of mice with mutation in LepR (db/db) and leptin (ob/ob) are not identical, and the cause remains unclear. Here, we show that db/db, but not ob/ob mice had defect in tenotomy-induced heterotopic ossification (HO), implicating alternative ligand(s) for LepR might be involved. Ligand screening revealed that ANGPTL4, a stress and fasting-induced factor, was elicited from brown adipose tissue after tenotomy, bound to LepR on PRRX1+ mesenchymal cells at the HO cite, thus promotes chondrogenesis and HO development. Disruption of LepR in PRRX1+ cells, or lineage ablation of LepR+ cells, or deletion of ANGPTL4 impeded chondrogenesis and HO in mice. Surprisingly, exogenous ANGPTL4 treatment not only promoted HO, but facilitated the transformation from white to brown adipose tissue in mice. These findings identify ANGPTL4 as a novel ligand for LepR to stimulate HO and regulate fat metabolism.

2024-07-03 | PXD043087 | Pride

Effect of heat stress on developing wheat grain

Project description:Effect of heat stress on developing wheat grain

| PRJEB35451 | ENA

Microarray analysis of heat and/or drought sensitivity of early grain development in wheat (Triticum aestivum L.)

Project description:Based on EST-based in silico gene expression analysis a 15k oligonucleotid microarray has been developped in order to monitor environmental stress-dependent gene expression changes in the wheat caryopsis. Using this array, the effect of water withdrawal, with and and without additional heat stress, during the first five days of grain development (0-5 DAA) has been investigated on two wheat cultivars differing in their drought sensitivity. The combined effect of heat and drought (DH) on gene expression was much significant (8-10% of the investigated genes changed >2-fold) in contrast to drought alone (1.5%). Drought and heat stress resulted in the co-ordinated change of the expression of storage proteins, some enzymes involved in sugar/starch metabolism, cell division-related and histone proteins, certain transcription factors, heat shock proteins, proteases and aquaporins. The potential link between the observed gene expression changes and the parallel histological observations indicating the accelerated development of the stressed grains is discussed.

2009-09-24 | GSE18205 | GEO

Transcriptome characterization of genotype-dependent effects of CaCl2 treatment on drought adaptation in barley (Hordeum vulgare L.).

Project description:The hypothesis whether CaCl2 seed treatment prior to sowing (osmopriming) can enhance drought stress tolerance, and/or alleviate the effect on plant growth was tested. Here we elucidate whether seed treatment prior to sowing (osmopriming) with CaCl2 can improve the drought stress tolerance, as it was previously reported for salt stress on wheat. Thus the effect of 50 mM CaCl2, previously chosen based on pilot experiment, versus water-treated control exposed to drought stress was investigated.

2015-09-30 | GSE56437 | GEO

E-MTAB-8520

Project description:Effect of heat stress on developing wheat grain

2020-01-10 | E-MTAB-8520 | ExpressionAtlas