Proteomics

Dataset Information

0

An Amphipathic Helix in Brl1 is Required for Nuclear Pore Complex Biogenesis in S. cerevisiae - KARMA analysis of Brl1 (DIA)

ABSTRACT: This project was aimed at analyzing the binding of Brl1 to the nuclear pore complex during the assembly process using the Kinetic Analysis of incorporation Rates in Macromolecular Assemblies (KARMA, Onischenko et al. 2020) method. In brief, we affinity purified Brl1 at several time points following SILAC metabolic labeling in growing S. cerevisiae cultures. Then, we analyzed the metabolic labeling for all co-purified nucleoporins and used these labeling kinetics to infer the timing of Brl1 binding during the native nuclear pore complex assembly.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Federico Uliana  

LAB HEAD: Federico Uliana

PROVIDER: PXD032017 | Pride | 2022-08-17

REPOSITORIES: pride

Json Xml

Dataset's files

Source:
Action DRS
210518_LU02_ulianaf_KW_05_09.raw Raw
210518_LU02_ulianaf_KW_05_10.raw Raw
210518_LU02_ulianaf_KW_05_11.raw Raw
210518_LU02_ulianaf_KW_05_13.raw Raw
210518_LU02_ulianaf_KW_05_14.raw Raw
Items per page:
1 - 5 of 20
altmetric image

Publications

The nuclear pore complex (NPC) is the central portal for macromolecular exchange between the nucleus and cytoplasm. In all eukaryotes, NPCs assemble into an intact nuclear envelope (NE) during interphase, but the process of NPC biogenesis remains poorly characterized. Furthermore, little is known about how NPC assembly leads to the fusion of the outer and inner NE, and no factors have been identified that could trigger this event. Here, we characterize the transmembrane protein Brl1 as an NPC as  ...[more]

Similar Datasets

Proteomics An Amphipathic Helix in Brl1 is Required for Nuclear Pore Complex Biogenesis in S. cerevisiae - KARMA analysis of Nup170 with/without Brl1 degradation (DIA)
2022-08-17 | PXD032024 | Pride
Proteomics An Amphipathic Helix in Brl1 is Required for Nuclear Pore Complex Biogenesis in S. cerevisiae - Lysate intermixing text (DIA)
2022-08-17 | PXD032034 | Pride
Proteomics An Amphipathic Helix in Brl1 is Required for Nuclear Pore Complex Biogenesis in S. cerevisiae – targeted analysis of cell lysate (PRM)
2022-08-17 | PXD032016 | Pride
Genomics The histone acetyltransferase Mst2 prevents epigenetic silencing via specific acetylation of the ubiquitin ligase Brl1 [microarray data]
2017-06-22 | GSE93433 | GEO
Transcriptomics The histone acetyltransferase Mst2 prevents epigenetic silencing via specific acetylation of the ubiquitin ligase Brl1 [NGS data]
2017-06-22 | GSE93432 | GEO
Proteomics,Multiomics The histone acetyltransferase Mst2 protects active chromatin from epigenetic silencing by acetylating the ubiquitin ligase Brl1
2017-06-28 | PXD005714 | Pride
Proteomics Geoduck (Panopea generosa) gonad LC-MS/MS DIA
2017-01-02 | PXD004921 | Pride
Genomics The histone acetyltransferase Mst2 prevents epigenetic silencing via specific acetylation of the ubiquitin ligase Brl1
| PRJNA360957 | ENA
Proteomics BoxCarmax: a novel high-selectivity data-independent acquisition mass spectrometry method for the analysis of protein turnover and complex samples
2021-02-02 | PXD021922 | Pride
Proteomics Subunit composition, molecular environment and activation of native TRPC channels encoded by their interactomes
2024-07-17 | PXD036019 | Pride