Dataset Information

Exploration of the nurse shark (Ginglymostoma cirratum) plasma immunoproteome using high-throughput LC-MS/MS

ABSTRACT: LC-MS/MS proteomics was used to identify immune proteins in the plasma of the nurse shark (Ginglymostoma cirratum), using a de novo multi-tissue transcriptome generated for this species. LC-MS/MS was then used to assess the host response to immunization with human serum albumin (HSA) and Complete Freund’s Adjuvant (CFA).

INSTRUMENT(S): Q Exactive

ORGANISM(S): Ginglymostoma Cirratum

TISSUE(S): Blood Plasma

SUBMITTER: David Stead

LAB HEAD: Dr Helen Dooley

PROVIDER: PXD032158 | Pride | 2022-08-12

REPOSITORIES: Pride

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Exploration of the Nurse Shark (<i>Ginglymostoma cirratum</i>) Plasma Immunoproteome Using High-Resolution LC-MS/MS.

Bakke Fiona K FK Gundappa Manu Kumar MK Matz Hanover H Stead David A DA Macqueen Daniel J DJ Dooley Helen H

Frontiers in immunology 20220606

Many animals of scientific importance lack species-specific reagents (e.g., monoclonal antibodies) for in-depth studies of immune proteins. Mass spectrometry (MS)-based proteomics has emerged as a useful method for monitoring changes in protein abundance and modifications in non-model species. It can be used to quantify hundreds of candidate immune molecules simultaneously without the generation of new reagents. Here, we used MS-based proteomics to identify and quantify candidate immune proteins ...[more]

PMID: 35734164

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