Ontology highlight
ABSTRACT:
INSTRUMENT(S): Orbitrap Fusion Lumos
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Hepatocyte, Cell Culture
SUBMITTER: wang hongwei
LAB HEAD: Gong Zhang
PROVIDER: PXD032201 | Pride | 2022-06-28
REPOSITORIES: Pride
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Wu Chun C Lu Xiaolong X Lu Shaohua S Wang Hongwei H Li Dehua D Zhao Jing J Jin Jingjie J Sun Zhenghua Z He Qing-Yu QY Chen Yang Y Zhang Gong G
Frontiers in molecular biosciences 20220602
Alternative splicing (AS) isoforms create numerous proteoforms, expanding the complexity of the genome. Highly similar sequences, incomplete reference databases and the insufficient sequence coverage of mass spectrometry limit the identification of AS proteoforms. Here, we demonstrated full-length translating mRNAs (ribosome nascent-chain complex-bound mRNAs, RNC-mRNAs) sequencing (RNC-seq) strategy to sequence the entire translating mRNA using next-generation sequencing, including short-read an ...[more]