Proteomics

Dataset Information

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Human hepatocellular carcinoma MHCC97H proteome


ABSTRACT: In this work, we generated a MHCC97H proteome datasets by In-gel digestion on Orbitrap Fusion Lumos. We systematically compared RNC-seq and Ribo-seq in the context of proteome identification, especially when identifying protein isoforms from AS. We also demonstrated that the single-molecule long read sequencing technique identified thousands of new splice variants and guided the MS identifications of new protein isoforms.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hepatocyte, Cell Culture

SUBMITTER: wang hongwei  

LAB HEAD: Gong Zhang

PROVIDER: PXD032201 | Pride | 2022-06-28

REPOSITORIES: Pride

Dataset's files

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Action DRS
20200102_LSH_97H_1.raw Raw
20200102_LSH_97H_10.raw Raw
20200102_LSH_97H_11.raw Raw
20200102_LSH_97H_12.raw Raw
20200102_LSH_97H_13.raw Raw
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Publications

Efficient Detection of the Alternative Spliced Human Proteome Using Translatome Sequencing.

Wu Chun C   Lu Xiaolong X   Lu Shaohua S   Wang Hongwei H   Li Dehua D   Zhao Jing J   Jin Jingjie J   Sun Zhenghua Z   He Qing-Yu QY   Chen Yang Y   Zhang Gong G  

Frontiers in molecular biosciences 20220602


Alternative splicing (AS) isoforms create numerous proteoforms, expanding the complexity of the genome. Highly similar sequences, incomplete reference databases and the insufficient sequence coverage of mass spectrometry limit the identification of AS proteoforms. Here, we demonstrated full-length translating mRNAs (ribosome nascent-chain complex-bound mRNAs, RNC-mRNAs) sequencing (RNC-seq) strategy to sequence the entire translating mRNA using next-generation sequencing, including short-read an  ...[more]

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