Proteomics

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Experimental maceration of bovine bones and its effects on the bone proteome

ABSTRACT: This study is aimed at understanding how bone soft maceration procedures (e.g., soaking the bones in warm water with the aid of detergents) used to process bones for long term storage (e.g., in human osteological collections) affect the bone proteome. Three different maceration protocols have been tested in the study, in order to test different lenghts for the soaking in water, different temperatures and different detergents. Bovine tibiae (n=6, 3 left and 3 right from 3 animals) have been used in the experiment - in particular, the left ones have been used as non-macerated controls, and the right ones have been macerated with three protocols (one per protocol). Sampling was conducted on both fresh and macerated remains and used to conduct shotgun proteomics analyses.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Bos Taurus (bovine)

TISSUE(S): Long Bone

SUBMITTER: Noemi Procopio  

LAB HEAD: Noemi Procopio

PROVIDER: PXD032295 | Pride | 2023-03-08

REPOSITORIES: Pride

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20210726_AP_NP_MSc_T1A.mgf Mgf
20210726_AP_NP_MSc_T1A.raw Raw
20210726_AP_NP_MSc_T1B.mgf Mgf
20210726_AP_NP_MSc_T1B.raw Raw
20210726_AP_NP_MSc_T2A.mgf Mgf
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Publications

The impact of maceration on the 'Osteo-ome'; a pilot investigation.

Gent Luke L   Schwalbe Edward C EC   Procopio Noemi N  

Journal of proteomics 20221013

The bone proteome, i.e., the 'osteo-ome', is a rich source of information for forensic studies. There have been advances in the study of biomolecule biomarkers for age-at-death (AAD) and post-mortem interval (PMI) estimations, by looking at changes in protein abundance and post-translational modifications (PTMs) at the peptide level. However, the extent to which other post-mortem factors alter the proteome, including 'maceration' procedures adopted in human taphonomy facilities (HTFs) to clean b  ...[more]

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