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Low-invasive sampling method for taxonomic for the identification of archaeological and paleontological bone by proteomics of their collagens

ABSTRACT: Bone collagen is an important organic material for isotopic measurement, radiocarbon and paleoproteomic analyzes, to provide information on diet, dating, taxonomic identification. Current paleoproteomics methods are destructive and require from a few milligrams to several tenths of milligrams of bone for analysis. In many cultures, bones are raw materials for artefact which are conserved in museum which hampers to damage these precious objects during sampling. Here, we describe a minimal sampling method that identifies collagen, taxonomy and post-translational modifications from Holocene and Upper Pleistocene bones dated to 130,000 and 5,000 years ago using dermatological skin tape-discs for sampling. The sampled bone micro-powder were digested following our highly optimized eFASP protocol, then analyzed by MALDI FTICR MS and LC-MS/MS for identifying the genus taxa of the bones. We show that this low-invasive sampling does not deteriorate the bones and achieves results similar to those obtained by destructive sampling. Moreover, this sampling method can be performed at archaeological sites or in museums.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Ursus Sp. Equus Sp. Cervus Elaphus (red Deer) Bos Taurus (bovine) Struthio Camelus (ostrich) Castor Fiber (eurasian Beaver) Capra Ibex (ibex) Sus Scrofa Domesticus (domestic Pig)

TISSUE(S): Bone

SUBMITTER: BRAY FABRICE

LAB HEAD: fabrice bray

PROVIDER: PXD044039 | Pride | 2024-07-25

REPOSITORIES: Pride

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