Dataset Information

Proteomic characterization of SEC-fractionated sEV subpopulations

ABSTRACT: The use of cardiac progenitor cell (CPC)-derived small extracellular vesicles (sEVs) has shown potential to stimulate cardiac repair. sEVs are released by cells and play a role in intercellular communication through transfer of their bioactive content. Increasing evidence indicates that sEVs present a heterogeneous population of vesicles. In the context of cardiac regeneration, studying sEV heterogeneity could provide new insights into mechanisms underlying sEV-mediated cardiac repair properties and help to improve the therapeutic application of CPC-derived sEVs. Here we used size-exclusion chromatography to isolate and fractionate EV subpopulations dervied from CPCs. We then applied a bottom-up proteomic approach to characterize the contents of individual EV subpopulations.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Stem Cell

SUBMITTER: Julia Bauzá Martinez

LAB HEAD: Wei Wu

PROVIDER: PXD032334 | Pride | 2024-01-26

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20220217_EX1_UM8_Bauza001_SA_EXT02_iso151_Unfraccionated_full.raw	Raw
	20220217_EX1_UM8_Bauza001_SA_EXT02_iso152_Unfraccionated_full.raw	Raw
	20220217_EX1_UM8_Bauza001_SA_EXT02_iso153_Unfraccionated_full.raw	Raw
	20220217_EX1_UM8_Bauza001_SA_EXT02_iso154_Unfraccionated_full.raw	Raw
	20220218_EX1_UM8_Bauza001_SA_EXT02_iso151_P1_full.raw	Raw

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Publications

Size matters: Functional differences of small extracellular vesicle subpopulations in cardiac repair responses.

van de Wakker Simonides Immanuel SI Bauzá-Martinez Julia J Ríos Arceo Carla C Manjikian Herak H Snijders Blok Christian Jamie Bernard CJB Roefs Marieke Theodora MT Willms Eduard E Maas Renee Goverdina Catharina RGC Pronker Matti Feije MF de Jong Olivier Gerrit OG Wu Wei W Görgens André A El Andaloussi Samir S Sluijter Joost Petrus Gerardus JPG Vader Pieter P

Journal of extracellular vesicles 20240101 1

Cardiac progenitor cell (CPC)-derived small extracellular vesicles (sEVs) exhibit great potential to stimulate cardiac repair. However, the multifaceted nature of sEV heterogeneity presents a challenge in understanding the distinct mechanisms underlying their regenerative abilities. Here, a dual-step multimodal flowthrough and size-exclusion chromatography method was applied to isolate and separate CPC-derived sEV subpopulations to study the functional differences related to cardiac repair respo ...[more]

PMID: 38179654

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