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Airway Epithelial Cell Response to Sendai virus infection


ABSTRACT: Oligonucleotide microarrays were used to establish a profile for gene expression in wild-type airway epithelial cells after paramyxoviral infection. Analysis was performed on mRNA isolated from SeV-infected primary-culture mouse tracheal epithelial cells that were maintained under physiologic conditions (air-liquid interface). Experiment Overall Design: Primary-culture mouse tracheal epithelial cells (mTECs) were established on Transwell membranes using air-liquid interface (ALI) conditions. Sendai virus (SeV), strain 52, was obtained from American Type Culture Collection and stored at -70°C. Cultures were inoculated with SeV or an equivalent amount of UV-inactivated SeV (SeV-UV) in the apical compartment for 1 h at 37 °C. Air-liquid-interface conditions were re-established by washing the membrane with PBS. Each culture well was subjected to one of two treatments (SeV, or UV-SeV) for 1 day. N = 4 SeV wells, N = 6 UV-SeV wells, with each well independently analyzed by microarray. No technical replicates were performed, but arrays were evaluated for quality control using the SimpleAffy package (Miller CJ, 2004) in Bioconductor 2.0.

ORGANISM(S): Mus musculus

SUBMITTER: Anand Champak Patel 

PROVIDER: E-GEOD-10211 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Airway epithelial versus immune cell Stat1 function for innate defense against respiratory viral infection.

Shornick Laurie P LP   Wells Audrey G AG   Zhang Yong Y   Patel Anand C AC   Huang Guangming G   Takami Kazutaka K   Sosa Moises M   Shukla Nikhil A NA   Agapov Eugene E   Holtzman Michael J MJ  

Journal of immunology (Baltimore, Md. : 1950) 20080301 5


The epithelial surface is often proposed to actively participate in host defense, but evidence that this is the case remains circumstantial. Similarly, respiratory paramyxoviral infections are a leading cause of serious respiratory disease, but the basis for host defense against severe illness is uncertain. Here we use a common mouse paramyxovirus (Sendai virus) to show that a prominent early event in respiratory paramyxoviral infection is activation of the IFN-signaling protein Stat1 in airway  ...[more]

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