Dataset Information

In vivo prenylomic profiling in the brain of a transgenic mouse model of Alzheimer’s disease reveals increased prenylation of a key set of proteins

ABSTRACT: Dysregulated prenylation has been implicated in the many diseases, including Alzheimer’s disease (AD). Prenylomic analysis, the combination of metabolic incorporation of an isoprenoid analogue (C15AlkOPP) into prenylated proteins with a bottom-up proteomic analysis, has allowed identification of prenylated proteins in various cellular models. Here, transgenic AD mice were treated with C15AlkOPP through intracerebroventricular (ICV) infusion over 13 days. Using prenylomic analysis, 37 prenylated proteins were enriched in the brains of AD mice . Importantly, the prenylated forms of 15 proteins were consistently upregulated in AD mice compared to non-transgenic wild-type controls. These results highlight the power of this in vivo metabolic labeling approach to identify multiple post-translationally modified proteins that may serve as potential therapeutic targets for a disease that has proved refractory to treatment thus far. Moreover, this method should be applicable to many other types of protein modifications, significantly broadening its scope.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain

DISEASE(S): Alzheimer's Disease

SUBMITTER: Shelby Auger

LAB HEAD: Mark, D, Distefano

PROVIDER: PXD032350 | Pride | 2022-10-28

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	1_WT_C15_profile_MQresults.zip	Other
	2APPVSWTproteinGroups.txt	Txt
	2_SVandC15_mzTab.mzTab	Mztab
	2_WT_C15andSVprofile_MQ_results.zip	Other
	3_APPVSWTproteinGroups.txt	Txt

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Publications

<i>In Vivo</i> Prenylomic Profiling in the Brain of a Transgenic Mouse Model of Alzheimer's Disease Reveals Increased Prenylation of a Key Set of Proteins.

Jeong Angela A Auger Shelby A SA Maity Sanjay S Fredriksen Kristina K Zhong Rui R Li Ling L Distefano Mark D MD

ACS chemical biology 20220915 10

Dysregulation of protein prenylation has been implicated in many diseases, including Alzheimer's disease (AD). Prenylomic analysis, the combination of metabolic incorporation of an isoprenoid analogue (C15AlkOPP) into prenylated proteins with a bottom-up proteomic analysis, has allowed the identification of prenylated proteins in various cellular models. Here, transgenic AD mice were administered with C15AlkOPP through intracerebroventricular (ICV) infusion over 13 days. Using prenylomic analysi ...[more]

PMID: 36109170

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