Dataset Information

Identification of novel target antigens in Membranous Nephorphathy

ABSTRACT: Membranous nephropathy (MN) is an autoimmune kidney disease with the clinical hallmark of causing high level proteinuria. MN is caused by circulating antibodies binding to targeting antigens on the surface of podocytes â€“ specialized endothelial cells contributing to the formation of the glomerular filtration barrier. Histomorphologically MN is characterized by a deposition of IgG along the glomerular basement membrane (GBM) â€“ predominantly IgG4 in primary MN â€“ as well as an accumulation of the respective target antigen, thickening of the GBM and effacement of podocyte foot processes. The predominant MN target antigen in 70-80% of MN patients is the phospholipase A2 receptor 1. Additional target antigens, which occur with lower frequencies, have recently been described. The aim of the study was the identification of a novel MN target antigen, which fulfills the typical criteria of MN target antigen: being a podocytic membrane protein, which is recognized by IgG4 subclass specific autoantibodies. Performing a mass spectrometry analysis based on a TMT-based relative quantification approach of immunoprecipitated samples resulted in the identification of Netrin G1 (NTNG1) as a novel MN target antigen. The results were validated using immunohistochemistry, Western Blot and ELISA. The molecular characterization of patients with MN will allow a better diagnosis and clinical management of these patients in the future.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Plasma, Kidney, Renal Glomerulus

DISEASE(S): Membranous Glomerulonephritis

SUBMITTER: Frank Stein

LAB HEAD: Prof. Rolf A.K. Stahl

PROVIDER: PXD032713 | Pride | 2022-08-03

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	F105687.dat	Other
	Lara_210312_P1572_JS_LR_TMT6_PD1of30_R1.raw	Raw
	P1572_Lara_210312_P1572_JS_LR_TMT6_PD1of30_R1_results_20210315_1428_peptides.txt	Txt
	P1572_Lara_210312_P1572_JS_LR_TMT6_PD1of30_R1_results_20210315_1428_proteins.txt	Txt
	P1572_Lara_210312_P1572_JS_LR_TMT6_PD1of30_R1_results_20210315_1428_summary.txt	Txt

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Publications

Netrin G1 Is a Novel Target Antigen in Primary Membranous Nephropathy.

Reinhard Linda L Machalitza Maya M Wiech Thorsten T Gröne Hermann-Josef HJ Lassé Moritz M Rinschen Markus M MM Ferru Nicoletta N Bräsen Jan Hinrich JH Drömann Friederike F Rob Peter Maria PM Sethi Sanjeev S Hoxha Elion E Stahl Rolf A K RAK

Journal of the American Society of Nephrology : JASN 20220819 10

<h4>Background</h4>Primary membranous nephropathy (MN) is caused by circulating autoantibodies binding to antigens on the podocyte surface. PLA<sub>2</sub>R1 is the main target antigen in 70%-80% of cases, but the pathogenesis is unresolved in 10%-15% of patients.<h4>Methods</h4>We used native western blotting to identify IgG4 autoantibodies, which bind an antigen endogenously expressed on podocyte membranes, in the serum of the index patient with MN. These IgG4 autoantibodies were used to immun ...[more]

PMID: 35985817

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