Proteomics

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Proteomic analysis of skeletal muscle from the Hybryid Mouse Diversity Panel

ABSTRACT: To begin to understand how variations in the genome drive changes in the skeletal muscle proteome, we performed a proteomic analysis of Gastrocnemius muscle from 73 inbred mouse strains of the Hybrid Mouse Diversity Panel (HMDP) that were fed a chow diet and housed under identical environmental conditions (n=2-4; 162 mice). These data were integrated with genomic and various molecular/phenotypic data via systems genetic analysis which is a powerful forward genetics approach to identify potential new regulators of complex traits. Proteomics was performed with eighteen 10-plex tandem mass tags (TMT) experiments each consisting of 9 strains plus a pooled common internal reference. Peptides were analysed by 2D-liquid chromatography coupled to tandem mass spectrometry (2D-LC-MS/MS) resulting in the quantification of 5,350 proteins with 4,027 quantified in >50 mice and 2,069 proteins quantified in all 162 animals.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Skeletal Muscle

SUBMITTER: Benjamin Parker  

LAB HEAD: Benjamin Parker

PROVIDER: PXD032729 | Pride | 2023-01-08

REPOSITORIES: Pride

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Dataset's files

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Action DRS
181103_HMDP_Muscle_SearchResults.rar Other
181105_Lumos_BenP_HMDP_Muscle_TMT1_F1.raw Raw
181105_Lumos_BenP_HMDP_Muscle_TMT1_F10.raw Raw
181105_Lumos_BenP_HMDP_Muscle_TMT1_F11.raw Raw
181105_Lumos_BenP_HMDP_Muscle_TMT1_F12.raw Raw
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Improving muscle function has great potential to improve the quality of life. To identify novel regulators of skeletal muscle metabolism and function, we performed a proteomic analysis of gastrocnemius muscle from 73 genetically distinct inbred mouse strains, and integrated the data with previously acquired genomics and >300 molecular/phenotypic traits via quantitative trait loci mapping and correlation network analysis. These data identified thousands of associations between protein abundance a  ...[more]

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