Dataset Information

Comparative proteomic of Trypanosoma cruzi strains

ABSTRACT: Trypanosoma cruzi, the causative agent of Chagas disease, is a genetically diverse with TcI being the most widespread lineage in the Americas. The protein expression dynamics allows unravel the biological phenomena of pathogens such as disease development and evolution. A large number of studies have shown a direct relationship between genetic variability and the biological characteristics of T. cruzi. However, much remains to be explored regarding the impact of gene expression on the development and evolution of Chagas disease. Two-dimensional (2D) electrophoresis and mass-spectrometry were employed to characterize the global protein expression patterns of epimastigotes from four distinct TcI strains, which display different growth kinetics. Biological differences were identified and confirmed throug metabolic and morphometric assays. Statistical tests (parametric or non-parametric test, as appropriate for the experiment) were used to assess the significance of the observed differences. The Ascending hierarchical classification analysis shows two clusters, that were congruent with their growth kinetics. The proteins expressed in one or the other cluster were identified by mass spectrometry. These proteins were evaluated by independent biological assays, which showed that the parasite’s metabolic activity and morphology is significantly different between the two clusters. We conclude that the protein expression patterns displayed by epimastigotes from fast/slow growth TcI strains are correlated with various biological parameters, including intensity of metabolic activity, metabolic fuel usage and resistance to oxidative stress, as well as morphological characteristics, such as length of the flagellum.

INSTRUMENT(S): LTQ Orbitrap XL

ORGANISM(S): Trypanosoma Cruzi

TISSUE(S): Cell Suspension Culture, Cell Culture

DISEASE(S): Chagas Disease

SUBMITTER: Functional Proteomics Platform FPP

LAB HEAD: Jaime Alfredo Costales Cordero

PROVIDER: PXD032748 | Pride | 2023-07-12

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	VNV_141002_HUMAN_X_408.msf	Msf
	VNV_141002_HUMAN_X_408.pep.xml	Pepxml
	VNV_141002_HUMAN_X_408.raw	Raw
	VNV_141002_HUMAN_X_411.msf	Msf
	VNV_141002_HUMAN_X_411.pep.xml	Pepxml

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Publications

Comparative proteomic analysis of Trypanosoma cruzi TcI lineage epimastigotes unveils metabolic and phenotypic differences between fast- and slow-dividing strains.

Telleria Jenny J Tibayrenc Michel M Del Salto Mendoza Michelle M Seveno Martial M Costales Jaime A JA

Experimental parasitology 20230708

Trypanosoma cruzi, the causative agent of Chagas disease, is a genetically and phenotypically diverse species, divided into 5 main phylogenetic lineages (TcI to TcVI). TcI is the most widespread lineage in the Americas. Proteomics is a suitable tool to study the global protein expression dynamics in pathogens. Previous proteomic studies have revealed a link between (i) the genetic variability; (ii) the protein expression; and (iii) the biological characteristics of T. cruzi. Here, two-dimensiona ...[more]

PMID: 37429537

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