Proteomics

Dataset Information

0

Identification of O-GlcNAcylated proteins in the protozoan parasite Trypanosoma cruzi


ABSTRACT: Trypanosoma cruzi is the causal agent of Chagas’ disease, which is one of the biggest public health problems in Latin America. Several post-translational modifications (PTM) have been studied in T. cruzi but there is no work focused on the O-GlcNAcylation, which is a highly conserved monosaccharide PTM found on serine and threonine residues of proteins mainly from the nucleus, cytoplasm and mitochondria. It is thought to regulate proteins in a manner analogous to protein phosphorylation, even their crosstalk allows the regulation of cellular functions in response to nutrients and stress. In the present work we demonstrate O-GlcNacylation in T. cruzi epimastigotes using two different methods: western blot with specific antibodies and click chemistry labelling. Then, we identify 2033 O-GlcNAcilated proteins and 48 modification sequences by mass spectrometry. Most of this proteins are part of structures, functions and pathways that are essentials for the parasite survival and evolution. For the first time O-GlcNacylation is idenytified in T. cruzi proteins and due to the many biological processes in which they participate, this study opens a new research field in Trypanosomatids biology, infection processes and potential identification of therapeutic targets.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Trypanosoma Cruzi Strain Cl Brener

SUBMITTER: Luc Camoin  

LAB HEAD: Luc Camoin

PROVIDER: PXD010285 | Pride | 2019-04-18

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
NR1ulsur25ul.mgf Mgf
NR1ulsur25ul.raw Raw
NR4ulsur25.mgf Mgf
NR4ulsur25.raw Raw
NR4ulsur25_180215191328.mgf Mgf
Items per page:
1 - 5 of 14
altmetric image

Publications


Originally an anthropozoonosis in the Americas, Chagas disease has spread from its previous borders through migration. It is caused by the protozoan <i>Trypanosoma cruzi</i>. Differences in disease severity have been attributed to a natural pleomorphism in <i>T. cruzi</i>. Several post-translational modifications (PTMs) have been studied in <i>T. cruzi</i>, but to date no work has focused on O-GlcNAcylation, a highly conserved monosaccharide-PTM of serine and threonine residues mainly found in n  ...[more]

Similar Datasets

2018-08-21 | PXD004860 | Pride
2023-08-03 | PXD039536 | Pride
2009-12-31 | E-GEOD-18130 | biostudies-arrayexpress
2010-01-01 | E-GEOD-18131 | biostudies-arrayexpress
2022-02-11 | PXD026495 | Pride
2024-07-15 | PXD053909 | Pride
2019-09-16 | PXD009731 | Pride
2024-05-21 | PXD045860 | Pride
2015-09-22 | PXD002208 | Pride
2023-10-20 | PXD045316 | Pride