Dataset Information

Human foreskin proteome with aSTI

ABSTRACT: There is scant data on the mechanisms through which asymptomatic STIs (aSTIs) alter risk of HIV acquisition in the male genital tract. Foreskin removal lowers the risk of HIV acquisition, but molecular events leading to this protection are unclear. Here we show that an asymptomatic urethral infection with Chlamydia trachomatis significantly alters the foreskin epithelial proteome composition. We developed and optimised a shotgun liquid chromatography coupled tandem mass spectrometry based proteomics approach and applied this to foreskins collected at the time of medical male circumcision from 16 aSTI+ cis-gender men and 10 age-matched STI- controls. We used a novel bioinformatic metaproteomic pipeline to detect differentially expressed proteins. Gene enrichment ontology analysis revealed proteins associated with inflammatory and immune activation function in both inner and outer foreskin from men with an aSTI. Neutrophil activation/degranulation and viral-evasion proteins were significantly enriched in foreskins from men with aSTI whereas homotypic cell-cell adhesion proteins were enriched in foreskin tissue from men without an STI. Collectively, our data show that an asymptomatic urethral sexually transmitted infection result in profound alterations in epitheial tissue that potentially causes depletion of barrier integrity and function and that may encourage HIV susceptibilty.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Skin

SUBMITTER: Nyaradzo Chigorimbo-Tsikiwa

LAB HEAD: Nyaradzo Chigorimbo-Tsikiwa

PROVIDER: PXD032798 | Pride | 2023-03-11

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Combined_Uniprot_human_2017_Nyari_TheThys_proteinGroups.txt	Txt
	NC_2016-07-21_C1_4_O.raw	Raw
	NC_2016-07-21_C1_5_I.raw	Raw
	NC_2016-07-21_C1_5_O.raw	Raw
	NC_2016-07-21_C1_6_I_160726165522.raw	Raw

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Publications

A pilot study to show that asymptomatic sexually transmitted infections alter the foreskin epithelial proteome.

Chigorimbo-Murefu Nyaradzo T L NTL Potgieter Matthys M Dzanibe Sonwabile S Gabazana Zikhona Z Buri Gershom G Chawla Aditya A Nleya Bokani B Olivier Abraham J AJ Harryparsad Rushil R Calder Bridget B Garnett Shaun S Maziya Lungile L Lewis David A DA Jaspan Heather H Wilson Doug D Passmore Jo-Ann S JS Mulder Nicola N Blackburn Jonathan J Bekker Linda-Gail LG Gray Clive M CM

Frontiers in microbiology 20221017

There is limited data on the role of asymptomatic STIs (aSTIs) on the risk of human immunodeficiency virus (HIV) acquisition in the male genital tract (MGT). The impact of foreskin removal on lowering HIV acquisition is well described, but molecular events leading to HIV acquisition are unclear. Here, in this pilot study, we show that asymptomatic urethral infection with <i>Chlamydia trachomatis</i> (CT) significantly impacts the foreskin proteome composition. We developed and optimized a shotgu ...[more]

PMID: 36325020

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