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Combining recombinase-mediated cassette exchange strategy with quantitative proteomic and phosphoproteomic analyses to inspect intracellular functions of the tumor supressor galectin-4 in colorectal cancer cells.

ABSTRACT: In order to systematically explore Galectin-4 function in CRC we established a CRC cell line where Gal4 expression can be regulated via doxycycline (dox)-inducible expression of a single copy wildtype LGALS4 transgene generated by recombinase-mediated cassette exchange (RMCE). Using this model and applying in depth proteomic and phosphoproteomic analyses we systematically screened for intracellular changes induced by Gal4 expression by.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Colon

DISEASE(S): Colon Cancer

SUBMITTER: Malwina Michalak

LAB HEAD: Juergen Kopitz

PROVIDER: PXD032902 | Pride | 2022-08-12

REPOSITORIES: Pride

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			Action	DRS
	MM0512_01-03_MQ_output.rar	Other
	MM0512_04-07_MQ_output.rar	Other
	checksum.txt	Txt
	oecf3-MM0512-01.raw	Raw
	oecf3-MM0512-02.raw	Raw

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Publications

Combining Recombinase-Mediated Cassette Exchange Strategy with Quantitative Proteomic and Phosphoproteomic Analyses to Inspect Intracellular Functions of the Tumor Suppressor Galectin-4 in Colorectal Cancer Cells.

Michalak Malwina M Golde Viola V Helm Dominik D Kaltner Herbert H Gebert Johannes J Kopitz Jürgen J

International journal of molecular sciences 20220608 12

Galectin-4 (Gal4) has been suggested to function as a tumor suppressor in colorectal cancer (CRC). In order to systematically explore its function in CRC, we established a CRC cell line where Gal4 expression can be regulated via the doxycycline (dox)-inducible expression of a single copy wildtype <i>LGALS4</i> transgene generated by recombinase-mediated cassette exchange (RMCE). Using this model and applying in-depth proteomic and phosphoproteomic analyses, we systematically screened for intrace ...[more]

PMID: 35742860

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