Proteomics

Dataset Information

0

Lactiplantibacillus plantarum, LC-MSMS


ABSTRACT: This project uses TMT labeling quantitative proteomics technology to carry out research, and a total of 898 proteins have been identified. Differentially expressed proteins were screened according to the criteria of expression fold change of more than 1.5-fold (up-regulation more than 1.5-fold or down-regulation less than 0.67) and P value<0.05. Among them, taking the comparison group Control VS H2O2 as an example, there were 31 up-regulated differentially expressed proteins and 81 down-regulated differentially expressed proteins. Through GO enrichment and KEGG pathway analysis, it was found that these differentially expressed proteins are mainly involved in important biological processes such as single-organism metabolic process, small molecule metabolic process, organophosphate metabolic process, organophosphate biosynthetic process and carbohydrate derivative biosynthetic process, and are mainly involved in the regulation of Metabolic pathways, Fructose and mannose metabolism, Oxidative phosphorylation, Tyrosine and Degradation of aromatic compounds and other important KEGG metabolic pathways.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Lactobacillus Plantarum Wcfs1

TISSUE(S): Cell Culture

SUBMITTER: Yuan Tian  

LAB HEAD: Yuhua Wang

PROVIDER: PXD032910 | Pride | 2022-08-12

REPOSITORIES: Pride

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Publications

Antioxidant Mechanism of <i>Lactiplantibacillus plantarum</i> KM1 Under H<sub>2</sub>O<sub>2</sub> Stress by Proteomics Analysis.

Tian Yuan Y   Wang Yu Y   Zhang Nan N   Xiao Minmin M   Zhang Jing J   Xing Xinyue X   Zhang Yue Y   Fan Yuling Y   Li Xia X   Nan Bo B   Wang Yuhua Y   Liu Jingsheng J  

Frontiers in microbiology 20220627


<i>Lactiplantibacillus plantarum</i> KM1 was screened from natural fermented products, which had probiotic properties and antioxidant function. The survival rate of <i>L. plantarum</i> KM1 was 78.26% at 5 mM H<sub>2</sub>O<sub>2</sub>. In this study, the antioxidant mechanism of <i>L. plantarum</i> KM1 was deeply analyzed by using the proteomics method. The results demonstrated that a total of 112 differentially expressed proteins (DEPs) were screened, of which, 31 DEPs were upregulated and 81 w  ...[more]

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