Proteomics

Dataset Information

0

SARS-CoV-2 phosphoproteomics - ACE2-A549 and Vero


ABSTRACT: Multiple coronavirus have emerged independently in the past 20 years and caused lethal human diseases. Although vaccine development targeting these viruses has been accelerated substantially, there remain patients requiring treatment who cannot be vaccinated. Understanding the common host factors necessary for the life cycle of coronaviruses may reveal conserved therapeutic targets. Here, we used the known substrate specificities of mammalian protein kinases to deconvolute the sequence of phosphorylation events mediated by three host protein kinase families (SRPK, GSK-3, and CK1) that phosphorylate a cluster of serine and threonine residues on the viral N-protein in a highly coordinated way. We showed that these kinases were critical for the viral replication cycle, suggesting that inhibitors of one or more of these protein kinases could be useful for treating patients with COVID-19 infections. These phosphorylation sites are highly conserved across coronaviruses, indicating that inhibitors of these protein kinases might be broadly effective in treating multiple viral diseases.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human) Chlorocebus Sabaeus

TISSUE(S): Lung, Epithelial Cell, Kidney

DISEASE(S): Covid-19

SUBMITTER: Tomer Yaron  

LAB HEAD: Lewis C. Cantley

PROVIDER: PXD033015 | Pride | 2023-03-11

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
052720_Cov2_CL_P1_total_1.raw Raw
052720_Cov2_CL_P1_total_10.raw Raw
052720_Cov2_CL_P1_total_11.raw Raw
052720_Cov2_CL_P1_total_12.raw Raw
052720_Cov2_CL_P1_total_2.raw Raw
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Publications

Host protein kinases required for SARS-CoV-2 nucleocapsid phosphorylation and viral replication.

Yaron Tomer M TM   Heaton Brook E BE   Levy Tyler M TM   Johnson Jared L JL   Jordan Tristan X TX   Cohen Benjamin M BM   Kerelsky Alexander A   Lin Ting-Yu TY   Liberatore Katarina M KM   Bulaon Danielle K DK   Van Nest Samantha J SJ   Koundouros Nikos N   Kastenhuber Edward R ER   Mercadante Marisa N MN   Shobana-Ganesh Kripa K   He Long L   Schwartz Robert E RE   Chen Shuibing S   Weinstein Harel H   Elemento Olivier O   Piskounova Elena E   Nilsson-Payant Benjamin E BE   Lee Gina G   Trimarco Joseph D JD   Burke Kaitlyn N KN   Hamele Cait E CE   Chaparian Ryan R RR   Harding Alfred T AT   Tata Aleksandra A   Zhu Xinyu X   Tata Purushothama Rao PR   Smith Clare M CM   Possemato Anthony P AP   Tkachev Sasha L SL   Hornbeck Peter V PV   Beausoleil Sean A SA   Anand Shankara K SK   Aguet François F   Getz Gad G   Davidson Andrew D AD   Heesom Kate K   Kavanagh-Williamson Maia M   Matthews David A DA   tenOever Benjamin R BR   Cantley Lewis C LC   Blenis John J   Heaton Nicholas S NS  

Science signaling 20221025 757


Multiple coronaviruses have emerged independently in the past 20 years that cause lethal human diseases. Although vaccine development targeting these viruses has been accelerated substantially, there remain patients requiring treatment who cannot be vaccinated or who experience breakthrough infections. Understanding the common host factors necessary for the life cycles of coronaviruses may reveal conserved therapeutic targets. Here, we used the known substrate specificities of mammalian protein  ...[more]

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