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Quantitative proteomic analysis of phosphorylated proteins during anoxia and reoxygenation in the anoxia-tolerant crucian carp

ABSTRACT: The freshwater fish crucian carp (Carassius carassius) are able to survive chronic anoxia for several months at low temperatures. Consequently, anoxia-related physiological and biochemical adaptations in this species have been studied for more than half a century. Still, despite for the well-known role of protein phosphorylation in regulating cellular processes, no studies have comprehensively characterized the phosphoproteome in crucian carp. In this study, we report the global phosphoproteome in crucian carp brain and liver during anoxia and reoxygenation. By applying a bottom-up proteomic approach on enriched phosphopeptides we found that the brain phosphoproteome show surprisingly few changes during anoxia-reoxygenation exposure with 110 out of 4316 phosphopeptides being differentially regulated. By contrast, in the liver 395 out of 1293 phosphopeptides were regulated. Although most changes occurred in the liver phosphoproteome, we found evidence for metabolic depression and decreased translation in both brain and liver. We also found regulated phosphoproteins involved in apoptotic regulation and reactive oxygen species handling in both tissues. In the brain, some of the most regulated phosphopeptides belonged to proteins involved in central nervous system development and neuronal activity at the synaptic cleft. Regulated phosphoproteins specific for liver tissue were related to glucose metabolism, including glycolytic flux and glycogenolysis, and the ubiquitin-proteasome system.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Carassius Carassius

TISSUE(S): Brain, Liver

SUBMITTER: Bernd Thiede

LAB HEAD: Anette Johansen

PROVIDER: PXD033061 | Pride | 2024-05-29

REPOSITORIES: Pride

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			Action	DRS
	BT_191118_AnetteJ_01.mgf	Mgf
	BT_191118_AnetteJ_01.raw	Raw
	BT_191118_AnetteJ_02.mgf	Mgf
	BT_191118_AnetteJ_02.raw	Raw
	BT_191118_AnetteJ_03.mgf	Mgf

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