Athaliana light response with TMT labeling

ABSTRACT: Incident light is a central modulator of plant growth and development. However, there are still open questions surrounding wavelength-specific plant proteomic responses. Here we applied tandem mass tag (TMT) based on quantitative proteomics technology to acquire an in-depth view of proteome profile changes in Arabidopsis thaliana response to treatment with narrow wavelength blue (B; 450 nm), amber (A; 595 nm), or red (R; 650 nm) light. A total of 16,707 proteins were identified with 9,120 proteins quantified across all three light treatments in three biological replicates (9 samples in total). This enabled examination of changes in the abundance for proteins expressed at lower levels with important regulatory roles, including transcription factors and hormone signaling. Importantly, 18% (1,631 proteins) of the A. thaliana proteome is differentially abundant in response to narrow wavelength lights, and changes in proteome correlate with different morphologies exhibited by plants in these experimental groups. This high-resolution resource for A. thaliana provides baseline data and a tool for defining molecular mechanisms that control fundamental aspects of a plant’s response to light wavelengths, with implications in plant development and adaptation.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Leaf

SUBMITTER: Nafiseh Yavari  

LAB HEAD: Nafiseh Yavari

PROVIDER: PXD033070 | Pride | 2022-07-11

REPOSITORIES: Pride