Dataset Information

Proteomic analysis of human neuronal SH-SY5Y in neurological diseases exposed to phtalates and metaks

ABSTRACT: This study aimed to reveal biological pathways involved in neurodevelopmental disorders after co-exposure to phthalates and metals by using a multi-omics analysis in a human neuronal cell line, SH-SY5Y. Mixtures of phthalates (DEHP, DiNP, BBzP) and metals (lead, methylmercury, total mercury, cadmium) were selected as they were the most abundant ones in the REPRO_PL and PHIME cohorts, respectively, in the frame of the EU project HEALS, investigating if exposure to those pollutants could be associated to neurodevelopmental disorders in children across Europe. Their concentrations in the human brain were calculated at the INTEGRA computational platform. The differentiated cell line was exposed to this mixture and a ten-times higher one for 4 days. Transcriptomic and proteomic analyses were performed and the data analyzed using advanced bioinformatics tools. Co-exposure to phthalates and metals leads to the perturbation of various metabolic pathways associated with neuronal diseases. The proteomic data showed that a number of neurological diseases are statistically associated with the exposure of the SH-SY5Y cells to the mixtures of pollutants. In conclusion, this study reveals that co-exposure to phthalates and metals disturbs biochemical processes in the brain model, which could be associated with clinical neurodevelopmental perturbations in cohorts.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Chiara guerrera

LAB HEAD: Chiara Guerrera

PROVIDER: PXD033091 | Pride | 2023-12-20

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	200124_HpH-LFQ_MaAG_Cont1-1_HpH1_Q31464.raw	Raw
	200124_HpH-LFQ_MaAG_Cont1-1_HpH2_Q31465.raw	Raw
	200124_HpH-LFQ_MaAG_Cont1-1_HpH3_Q31466.raw	Raw
	200124_HpH-LFQ_MaAG_Cont1-1_HpH4_Q31467.raw	Raw
	200124_HpH-LFQ_MaAG_Cont2-1_HpH1_Q31468.raw	Raw

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Publications

Aggressiveness and Metastatic Potential of Breast Cancer Cells Co-Cultured with Preadipocytes and Exposed to an Environmental Pollutant Dioxin: An <i>in Vitro</i> and <i>in Vivo</i> Zebrafish Study.

Koual Meriem M Tomkiewicz Céline C Guerrera Ida Chiara IC Sherr David D Barouki Robert R Coumoul Xavier X

Environmental health perspectives 20210308 3

<h4>Background</h4>Breast cancer (BC) is a major public health concern, and its prognosis is very poor once metastasis occurs. The tumor microenvironment and chemical pollution have been suggested recently to contribute, independently, to the development of metastatic cells. The BC microenvironment consists, in part, of adipocytes and preadipocytes in which persistent organic pollutants (POPs) can be stored.<h4>Objectives</h4>We aimed to test the hypothesis that these two factors (2,3,7,8-tetrac ...[more]

PMID: 33683140

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