Proteomics

Dataset Information

0

Quantitative analysis of redox proteome reveals protein thiol switches regulating fundamental processes in developmental hematopoiesis


ABSTRACT: Fetal and adult hematopoietic stem and progenitor cells (HSPCs) are characterized by distinct redox homeostasis that may influence their differential cellular behaviour in normal and malignant haematopoiesis. In this work, we have applied a quantitative mass spectrometry-based redox proteomic approach to comprehensively describe reversible cysteine modifications in primary mouse fetal and adult HSPCs. We defined the redox state of 4455 cysteines in fetal and adult HSPCs and demonstrated a higher susceptibility to oxidation of protein thiols in fetal HSPCs. Our data identified ontogenically active redox switches in proteins with a pronounced role in metabolism and protein homeostasis. Additional redox proteomic analysis identified redox switches acting in mitochondrial respiration as well as protein homeostasis to be triggered during onset of MLL-ENL leukemogenesis in fetal HSPCs. Our data has demonstrated that redox signalling contributes to the regulation of fundamental processes of developmental hematopoiesis and has pinpointed potential targetable redox-sensitive proteins in in utero-initiated MLL-rearranged leukaemia. An H9 human embryonic stem cells cell line was applied to validate data from the primary cells.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Embryonic Stem Cell

SUBMITTER: Kristyna Pimkova  

LAB HEAD: Kristyna Pimkova

PROVIDER: PXD033115 | Pride | 2022-06-09

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
KP127_H2O2_E_4ul_01.raw Raw
KP127_H2O2_Full_4ul_01.raw Raw
KP134_Rep2_E_6ul_01.raw Raw
KP134_Rep2_FT_2ul_01.raw Raw
KP134_Rep3_E_6ul_01.raw Raw
Items per page:
1 - 5 of 11
altmetric image

Publications

Quantitative analysis of redox proteome reveals oxidation-sensitive protein thiols acting in fundamental processes of developmental hematopoiesis.

Pimkova K K   Jassinskaja M M   Munita R R   Ciesla M M   Guzzi N N   Cao Thi Ngoc P P   Vajrychova M M   Johansson E E   Bellodi C C   Hansson J J  

Redox biology 20220523


Fetal and adult hematopoietic stem and progenitor cells (HSPCs) are characterized by distinct redox homeostasis that may influence their differential cellular behavior in normal and malignant hematopoiesis. In this work, we have applied a quantitative mass spectrometry-based redox proteomic approach to comprehensively describe reversible cysteine modifications in primary mouse fetal and adult HSPCs. We defined the redox state of 4,438 cysteines in fetal and adult HSPCs and demonstrated a higher  ...[more]

Similar Datasets

2022-06-09 | PXD030043 | Pride
2024-04-02 | PXD042249 | Pride
2023-10-29 | E-MTAB-12806 | biostudies-arrayexpress
2015-08-27 | E-GEOD-69760 | biostudies-arrayexpress
2021-12-30 | PXD027550 | Pride
2012-10-05 | E-GEOD-36984 | biostudies-arrayexpress
2024-04-02 | PXD049014 | Pride
2021-06-26 | GSE178951 | GEO
2015-08-27 | GSE69760 | GEO
2019-07-16 | GSE125549 | GEO