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N-linked glycan analysis of native SARS-CoV-2 Delta variant Spike

ABSTRACT: The SARS-CoV-2 Delta variant is more contagious than WT and causes severe symptoms.To understand the elevated fusogenicity of Delta, we used LC-MS/MS to characterize the site specific N-linked glycan of spike protein in SARS-CoV-2 Delta variant virions.The glycan analysis revealed increased oligomannose-type glycosylation of native Delta spike over that of the Wuhan-Hu-1 spike.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Severe Acute Respiratory Syndrome Coronavirus 2

TISSUE(S): Cell Culture

SUBMITTER: Yutong Song

LAB HEAD: Sai Li

PROVIDER: PXD033342 | Pride | 2023-05-10

REPOSITORIES: Pride

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			Action	DRS
	MS212156-VERO-S-1-T-C.raw	Raw
	MS212156-vero-s-1-T_C-delta-noenzyme.zip	Other
	MS212156-vero-s-2-lytic-delta.zip	Other
	MS212156-vero-s-2-lytic.raw	Raw

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Publications

In situ architecture and membrane fusion of SARS-CoV-2 Delta variant.

Song Yutong Y Yao Hangping H Wu Nanping N Xu Jialu J Zhang Zheyuan Z Peng Cheng C Li Shibo S Kong Weizheng W Chen Yong Y Zhu Miaojin M Wang Jiaqi J Shi Danrong D Zhao Chongchong C Lu Xiangyun X Echavarría Galindo Martín M Li Sai S

Proceedings of the National Academy of Sciences of the United States of America 20230424 18

Among the current five Variants of Concern, infections caused by SARS-CoV-2 B.1.617.2 (Delta) variant are often associated with the greatest severity. Despite recent advances on the molecular basis of elevated pathogenicity using recombinant proteins, the architecture of intact Delta virions remains veiled. Moreover, pieces of molecular evidence for the detailed mechanism of S-mediated membrane fusion are missing. Here, we showed the pleomorphic nature of Delta virions from electron beam inactiv ...[more]

PMID: 37094167

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