Proteomics

Dataset Information

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Label-free whole cell proteomics for oncogene cell lines

ABSTRACT: Label-free whole cell proteomics was performed for the following cell lines: LHS-PREC engineered to overexpress MYC or EV; P4936 overexpressing MYC on a tetracycline-repressible promotor; MCF10A cells expressing signal transduction oncogenes AKT, BRAF, EGFR, HER2, KRAS, MEK; KP4 and PSN1 PDAC cell lines vs HPDE normal pancreatic cell; PDX-derived osteosarcoma cell lines with MYC amplification vs hFOB cell

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Pancreatic Ductal Cell, B Cell, Osteoblast, Breast, Cell Culture, Prostate Epithelium

DISEASE(S): Osteosarcoma,Prostate Cancer,Burkitt Lymphoma,Pancreatic Cancer

SUBMITTER: Paige Solomon  

LAB HEAD: Jim Wells

PROVIDER: PXD033373 | Pride | 2022-08-11

REPOSITORIES: Pride

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20180506_GMW_Wells_MCF_AKT_Depleted_1.raw Raw
20180506_GMW_Wells_MCF_AKT_Depleted_2.raw Raw
20180506_GMW_Wells_MCF_AKT_Depleted_3.raw Raw
20180506_GMW_Wells_MCF_Braf_Depleted_1.raw Raw
20180506_GMW_Wells_MCF_Braf_Depleted_2.raw Raw
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Publications

Discovery Proteomics Analysis Determines That Driver Oncogenes Suppress Antiviral Defense Pathways Through Reduction in Interferon-β Autocrine Stimulation.

Solomon Paige E PE   Kirkemo Lisa L LL   Wilson Gary M GM   Leung Kevin K KK   Almond Mark H MH   Sayles Leanne C LC   Sweet-Cordero E Alejandro EA   Rosenberg Oren S OS   Coon Joshua J JJ   Wells James A JA  

Molecular & cellular proteomics : MCP 20220518 7

Since the discovery of oncogenes, there has been tremendous interest to understand their mechanistic basis and to develop broadly actionable therapeutics. Some of the most frequently activated oncogenes driving diverse cancers are c-MYC, EGFR, HER2, AKT, KRAS, BRAF, and MEK. Using a reductionist approach, we explored how cellular proteomes are remodeled in isogenic cell lines engineered with or without these driver oncogenes. The most striking discovery for all oncogenic models was the systemati  ...[more]

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